Medical Microbiology and Immunology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Bartonella quintana and Bartonella vinsonii subsp. vinsonii bloodstream co-infection in a girl from North Carolina, USA
We report the first documented human case of blood stream infection withBartonella vinsonii subsp.vinsonii in a girl from North Carolina, USA, who was co-infected withBartonella quintana. Limitations ofBartonella spp. serology and the challenges of microbiological culture and molecular diagnostic confirmation of co-infection with more than oneBartonella spp. are discussed. When and where these infections were acquired is unknown; however, exposure to rodents, fleas and cats in the peri-equestrian environment was a suspected source for transmission of both organisms. (Source: Medical Microbiology and Immunology)
Source: Medical Microbiology and Immunology - September 24, 2018 Category: Microbiology Source Type: research

Correction to: Blocking of opioid receptors in experimental formaline-inactivated respiratory syncytial virus (FI-RSV) immunopathogenesis: from beneficial to harmful impacts
In the original publication, seventh author ’s name was incorrectly published as ‘Maryam Golaram’. (Source: Medical Microbiology and Immunology)
Source: Medical Microbiology and Immunology - September 22, 2018 Category: Microbiology Source Type: research

Intranasal co-administration of recombinant active fragment of Zonula occludens toxin and truncated recombinant EspB triggers potent systemic, mucosal immune responses and reduces span of E. coli O157:H7 fecal shedding in BALB/c mice
AbstractEscherichia coli O157:H7 with its traits such as intestinal colonization and fecal-oral route of transmission demands mucosal vaccine development.E. coli secreted protein B (EspB) is one of the key type III secretory system (TTSS) targets for mucosal candidate vaccine due to its indispensable role in the pathogenesis ofE. coli O157:H7. However, mucosally administered recombinant proteins have low immunogenicity which could be overcome by the use of mucosal adjuvants. The quest for safe, potent mucosal adjuvant has recognized ΔG fragment of Zonula occludens toxin ofVibrio cholerae with such properties. ΔG enhances...
Source: Medical Microbiology and Immunology - September 12, 2018 Category: Microbiology Source Type: research

Role of pentamer complex-specific and IgG subclass 3 antibodies in HCMV hyperimmunoglobulin and standard intravenous IgG preparations
ConclusionVia specific antibody depletion, we could demonstrate that pentameric complex-specific antibodies are present in HIG and bind to the recombinant PC resulting in a highly significant reduction of NT-capacity compared to the UL130 TAN-and SWS-peptides. We could not confirm the functional role of IgG subclass 3 neutralizing antibodies in IgG-preparations. (Source: Medical Microbiology and Immunology)
Source: Medical Microbiology and Immunology - September 10, 2018 Category: Microbiology Source Type: research

The sequence analysis of Epstein –Barr virus EBNA1 gene: could viral screening markers for nasopharyngeal carcinoma be identified?
AbstractEpstein –Barr virus (EBV) has been identified as a group 1 carcinogenic agent, particularly for nasopharyngeal carcinoma (NPC). The sequence diversity of EBV nuclear antigen 1 (EBNA1) reflects region-restricted polymorphisms, which may be associated with the development of certain malignancies. The aims o f the present study were to evaluate EBV EBNA1 gene polymorphisms circulating in NPC, infectious mononucleosis, and isolates from patients with transplanted organs to determine if EBNA1 sequence specificities are useful as viral biomarkers for NPC. Forty biopsies of undifferentiated carcinoma of nas opharyngeal ...
Source: Medical Microbiology and Immunology - September 10, 2018 Category: Microbiology Source Type: research

Role of pentamer complex-specific and IgG subclass 3 antibodies in HCMV hyperimmunoglobulin and standard intravenous IgG preparations
ConclusionVia specific antibody depletion, we could demonstrate that pentameric complex-specific antibodies are present in HIG and bind to the recombinant PC resulting in a highly significant reduction of NT-capacity compared to the UL130 TAN-and SWS-peptides. We could not confirm the functional role of IgG subclass 3 neutralizing antibodies in IgG-preparations. (Source: Medical Microbiology and Immunology)
Source: Medical Microbiology and Immunology - September 10, 2018 Category: Microbiology Source Type: research

The sequence analysis of Epstein –Barr virus EBNA1 gene: could viral screening markers for nasopharyngeal carcinoma be identified?
AbstractEpstein –Barr virus (EBV) has been identified as a group 1 carcinogenic agent, particularly for nasopharyngeal carcinoma (NPC). The sequence diversity of EBV nuclear antigen 1 (EBNA1) reflects region-restricted polymorphisms, which may be associated with the development of certain malignancies. The aims o f the present study were to evaluate EBV EBNA1 gene polymorphisms circulating in NPC, infectious mononucleosis, and isolates from patients with transplanted organs to determine if EBNA1 sequence specificities are useful as viral biomarkers for NPC. Forty biopsies of undifferentiated carcinoma of nas opharyngeal ...
Source: Medical Microbiology and Immunology - September 10, 2018 Category: Microbiology Source Type: research

Activation of brain endothelium by Escherichia coli K1 virulence factor cglD promotes polymorphonuclear leukocyte transendothelial migration
AbstractEscherichia coli K1 is the most common Gram-negative bacteria causing neonatal meningitis. Polymorphonuclear leukocyte (PMN) transmigration across the blood –brain barrier (BBB) is the hallmark of bacterial meningitis. Reportedly, the deletion of virulence factor cglD (E44:ΔcglD) from E44 is responsible for a less efficient PMN transendothelial migration ability. In the present study, we found that complementation of thecglD gene into E44: ΔcglD mutant strain might restore the PMN count and myeloperoxidase level in a neonatal mouse meningitis. Using human brain microvascular endothelial cells (HBMECs), the main...
Source: Medical Microbiology and Immunology - August 31, 2018 Category: Microbiology Source Type: research

Polymorphisms in the TGFB1 signal peptide influence human papillomavirus infection and development of cervical lesions
AbstractThe main purpose was to assess the effect of c.29C>T and c.74G>C polymorphisms in theTGFB1 signal peptide on HPV infection and development of cervical lesions. Cervical swabs and blood samples were obtained from 349 outpatient women, along with socio-demographic and sexual behavioral data. The study population was stratified by absence or presence of HPV DNA, as tested by PCR, as well as by lesion grade.TGFB1 signal peptide polymorphisms were genotyped using PCR-restriction fragment length polymorphism. HPV DNA was detected in 172 (49.3%) patients. c.74GC and the combined c.29CC+CT/c.74GC genotype were more f...
Source: Medical Microbiology and Immunology - August 30, 2018 Category: Microbiology Source Type: research

β-Glucan induces autophagy in dendritic cells and influences T-cell differentiation
In this study, we observed that β-glucan induced the expression of a number of autophagy-related genes and the formation of autophagosomes in DCs. To further investigate whether β-glucan-induced DC activation and innate cytokine production are associated with autophagy, we utilized 3-MA to block autophagosome formation and accessed the maturation and function of DCs induced by β-glucan. We found that autophagy-deficie nt DCs showed downregulated expression of MHC-II and CD80, decreased TNF-α secretion, and reduced production of iNOS upon β-glucan stimulation. Further examination demonstrated that blockade of autophagy...
Source: Medical Microbiology and Immunology - August 7, 2018 Category: Microbiology Source Type: research