Functional genomics of Brassica napus: Progresses, challenges, and perspectives
J Integr Plant Biol. 2024 Mar 8. doi: 10.1111/jipb.13635. Online ahead of print.ABSTRACTBrassica napus, commonly known as rapeseed or canola, is a major oil crop contributing over 13% to the stable supply of edible vegetable oil worldwide. Identification and understanding the gene functions in the B. napus genome is crucial for genomic breeding. A group of genes controlling agronomic traits have been successfully cloned through functional genomics studies in B. napus. In this review, we present an overview of the progress made in the functional genomics of B. napus, including the availability of germplasm resources, omics ...
Source: Journal of Integrative Plant Biology - March 8, 2024 Category: Biology Authors: Zengdong Tan Xu Han Cheng Dai Shaoping Lu Hanzi He Xuan Yao Peng Chen Chao Yang Lun Zhao Qing-Yong Yang Jun Zou Jing Wen Dengfeng Hong Chao Liu Xianhong Ge Chuchuan Fan Bing Yi Chunyu Zhang Chaozhi Ma Kede Liu Jinxiong Shen Jinxing Tu Guangsheng Yang Ting Source Type: research

Expansion and improvement of ChinaMu by MuT-seq and chromosome-level assembly of the Mu-starter genome
In this study, we made significant improvements to the size and quality of the ChinaMu library. We developed a new Mu-tag isolation method Mu-Tn5-seq (MuT-seq). Compared to the previous method used by ChinaMu, MuT-seq recovered 1/3 more germinal insertions, while requiring only about 1/14 of the sequencing volume and 1/5 of the experimental time. Using MuT-seq, we identified 113,879 germinal insertions from 3,168 Mu-active F1 families. We also assembled a high-quality genome for the Mu-active line Mu-starter, which harbors the initial active MuDR element and was used as the pollen donor for the mutation population. Using t...
Source: Journal of Integrative Plant Biology - March 7, 2024 Category: Biology Authors: Lei Liang Yuancong Wang Yanbin Han Yicong Chen Mengfei Li Yibo Wu Zeyang Ma Han Zhao Rentao Song Source Type: research

Molecular regulation of the key specialized metabolism pathways in medicinal plants
J Integr Plant Biol. 2024 Mar 5. doi: 10.1111/jipb.13634. Online ahead of print.ABSTRACTThe basis of modern pharmacology is the human ability to exploit the production of specialized metabolites from medical plants, for example, terpenoids, alkaloids, and phenolic acids. However, in most cases, the availability of these valuable compounds is limited by cellular or organelle barriers or spatio-temporal accumulation patterns within different plant tissues. Transcription factors (TFs) regulate biosynthesis of these specialized metabolites by tightly controlling the expression of biosynthetic genes. Cutting-edge technologies a...
Source: Journal of Integrative Plant Biology - March 5, 2024 Category: Biology Authors: Min Shi Siwei Zhang Zizhen Zheng Itay Maoz Lei Zhang Guoyin Kai Source Type: research

Molecular regulation of the key specialized metabolism pathways in medicinal plants
J Integr Plant Biol. 2024 Mar 5. doi: 10.1111/jipb.13634. Online ahead of print.ABSTRACTThe basis of modern pharmacology is the human ability to exploit the production of specialized metabolites from medical plants, for example, terpenoids, alkaloids, and phenolic acids. However, in most cases, the availability of these valuable compounds is limited by cellular or organelle barriers or spatio-temporal accumulation patterns within different plant tissues. Transcription factors (TFs) regulate biosynthesis of these specialized metabolites by tightly controlling the expression of biosynthetic genes. Cutting-edge technologies a...
Source: Journal of Integrative Plant Biology - March 5, 2024 Category: Biology Authors: Min Shi Siwei Zhang Zizhen Zheng Itay Maoz Lei Zhang Guoyin Kai Source Type: research

Molecular regulation of the key specialized metabolism pathways in medicinal plants
J Integr Plant Biol. 2024 Mar 5. doi: 10.1111/jipb.13634. Online ahead of print.ABSTRACTThe basis of modern pharmacology is the human ability to exploit the production of specialized metabolites from medical plants, for example, terpenoids, alkaloids, and phenolic acids. However, in most cases, the availability of these valuable compounds is limited by cellular or organelle barriers or spatio-temporal accumulation patterns within different plant tissues. Transcription factors (TFs) regulate biosynthesis of these specialized metabolites by tightly controlling the expression of biosynthetic genes. Cutting-edge technologies a...
Source: Journal of Integrative Plant Biology - March 5, 2024 Category: Biology Authors: Min Shi Siwei Zhang Zizhen Zheng Itay Maoz Lei Zhang Guoyin Kai Source Type: research

SlBEL11 regulates flavonoid biosynthesis, thus fine-tuning auxin efflux to prevent premature fruit drop in tomato
J Integr Plant Biol. 2024 Feb 29. doi: 10.1111/jipb.13627. Online ahead of print.ABSTRACTAuxin regulates flower and fruit abscission, but how developmental signals mediate auxin transport in abscission remains unclear. Here, we reveal the role of the transcription factor BEL1-LIKE HOMEODOMAIN11 (SlBEL11) in regulating auxin transport during abscission in tomato (Solanum lycopersicum). SlBEL11 is highly expressed in the fruit abscission zone, and its expression increases during fruit development. Knockdown of SlBEL11 expression by RNA interference (RNAi) caused premature fruit drop at the breaker (Br) and 3 d post-breaker (...
Source: Journal of Integrative Plant Biology - February 29, 2024 Category: Biology Authors: Xiufen Dong Xianfeng Liu Lina Cheng Ruizhen Li Siqi Ge Sai Wang Yue Cai Yang Liu Sida Meng Cai-Zhong Jiang Chun-Lin Shi Tianlai Li Daqi Fu Mingfang Qi Tao Xu Source Type: research

SlBEL11 regulates flavonoid biosynthesis, thus fine-tuning auxin efflux to prevent premature fruit drop in tomato
J Integr Plant Biol. 2024 Feb 29. doi: 10.1111/jipb.13627. Online ahead of print.ABSTRACTAuxin regulates flower and fruit abscission, but how developmental signals mediate auxin transport in abscission remains unclear. Here, we reveal the role of the transcription factor BEL1-LIKE HOMEODOMAIN11 (SlBEL11) in regulating auxin transport during abscission in tomato (Solanum lycopersicum). SlBEL11 is highly expressed in the fruit abscission zone, and its expression increases during fruit development. Knockdown of SlBEL11 expression by RNA interference (RNAi) caused premature fruit drop at the breaker (Br) and 3 d post-breaker (...
Source: Journal of Integrative Plant Biology - February 29, 2024 Category: Biology Authors: Xiufen Dong Xianfeng Liu Lina Cheng Ruizhen Li Siqi Ge Sai Wang Yue Cai Yang Liu Sida Meng Cai-Zhong Jiang Chun-Lin Shi Tianlai Li Daqi Fu Mingfang Qi Tao Xu Source Type: research

SlBEL11 regulates flavonoid biosynthesis, thus fine-tuning auxin efflux to prevent premature fruit drop in tomato
J Integr Plant Biol. 2024 Feb 29. doi: 10.1111/jipb.13627. Online ahead of print.ABSTRACTAuxin regulates flower and fruit abscission, but how developmental signals mediate auxin transport in abscission remains unclear. Here, we reveal the role of the transcription factor BEL1-LIKE HOMEODOMAIN11 (SlBEL11) in regulating auxin transport during abscission in tomato (Solanum lycopersicum). SlBEL11 is highly expressed in the fruit abscission zone, and its expression increases during fruit development. Knockdown of SlBEL11 expression by RNA interference (RNAi) caused premature fruit drop at the breaker (Br) and 3 d post-breaker (...
Source: Journal of Integrative Plant Biology - February 29, 2024 Category: Biology Authors: Xiufen Dong Xianfeng Liu Lina Cheng Ruizhen Li Siqi Ge Sai Wang Yue Cai Yang Liu Sida Meng Cai-Zhong Jiang Chun-Lin Shi Tianlai Li Daqi Fu Mingfang Qi Tao Xu Source Type: research

SlBEL11 regulates flavonoid biosynthesis, thus fine-tuning auxin efflux to prevent premature fruit drop in tomato
J Integr Plant Biol. 2024 Feb 29. doi: 10.1111/jipb.13627. Online ahead of print.ABSTRACTAuxin regulates flower and fruit abscission, but how developmental signals mediate auxin transport in abscission remains unclear. Here, we reveal the role of the transcription factor BEL1-LIKE HOMEODOMAIN11 (SlBEL11) in regulating auxin transport during abscission in tomato (Solanum lycopersicum). SlBEL11 is highly expressed in the fruit abscission zone, and its expression increases during fruit development. Knockdown of SlBEL11 expression by RNA interference (RNAi) caused premature fruit drop at the breaker (Br) and 3 d post-breaker (...
Source: Journal of Integrative Plant Biology - February 29, 2024 Category: Biology Authors: Xiufen Dong Xianfeng Liu Lina Cheng Ruizhen Li Siqi Ge Sai Wang Yue Cai Yang Liu Sida Meng Cai-Zhong Jiang Chun-Lin Shi Tianlai Li Daqi Fu Mingfang Qi Tao Xu Source Type: research

Orchestrating seed storage protein and starch accumulation toward overcoming yield-quality trade-off in cereal crops
J Integr Plant Biol. 2024 Feb 26. doi: 10.1111/jipb.13633. Online ahead of print.ABSTRACTAchieving high yield and good quality in crops is essential for human food security and health. However, there is usually disharmony between yield and quality. Seed storage protein (SSP) and starch, the predominant components in cereal grains, determine yield and quality, and their coupled synthesis causes a yield-quality trade-off. Therefore, dissection of the underlying regulatory mechanism facilitates simultaneous improvement of yield and quality. Here, we summarize current findings about the synergistic molecular machinery underpin...
Source: Journal of Integrative Plant Biology - February 27, 2024 Category: Biology Authors: Shuanghe Cao Bingyan Liu Daowen Wang Awais Rasheed Lina Xie Xianchun Xia Zhonghu He Source Type: research

Structural insights into the Oryza sativa cation transporters HKTs in salt tolerance
In this study, we elucidate the structures of OsHKT1;1 and OsHKT2;1, representing two distinct classes of rice HKTs. The dimeric assembled OsHKTs can be structurally divided into four domains. At the dimer interface, a half-helix or a loop in the third domain is coordinated by the C-terminal region of the opposite subunit. Additionally, we present the structures of OsHKT1;5 salt-tolerant and salt-sensitive variants, a key quantitative trait locus associated with salt tolerance. The salt-tolerant variant of OsHKT1;5 exhibits enhanced Na+ transport capability and displays a more flexible conformation. These findings shed lig...
Source: Journal of Integrative Plant Biology - February 27, 2024 Category: Biology Authors: Ran Gao Yutian Jia Xia Xu Peng Fu Jiaqi Zhou Guanghui Yang Source Type: research

Structural insights into the unusual core photocomplex from a triply extremophilic purple bacterium, Halorhodospira halochloris
J Integr Plant Biol. 2024 Feb 27. doi: 10.1111/jipb.13628. Online ahead of print.ABSTRACTHalorhodospira (Hlr.) halochloris is a triply extremophilic phototrophic purple sulfur bacterium, as it is thermophilic, alkaliphilic, and extremely halophilic. The light-harvesting-reaction center (LH1-RC) core complex of this bacterium displays an LH1-Qy transition at 1,016 nm, which is the lowest-energy wavelength absorption among all known phototrophs. Here we report the cryo-EM structure of the LH1-RC at 2.42 Å resolution. The LH1 complex forms a tricyclic ring structure composed of 16 αβγ-polypeptides and one αβ-heterodimer...
Source: Journal of Integrative Plant Biology - February 27, 2024 Category: Biology Authors: Chen-Hui Qi Guang-Lei Wang Fang-Fang Wang Jie Wang Xiang-Ping Wang Mei-Juan Zou Fei Ma Michael T Madigan Yukihiro Kimura Zheng-Yu Wang-Otomo Long-Jiang Yu Source Type: research

Orchestrating seed storage protein and starch accumulation toward overcoming yield-quality trade-off in cereal crops
J Integr Plant Biol. 2024 Feb 26. doi: 10.1111/jipb.13633. Online ahead of print.ABSTRACTAchieving high yield and good quality in crops is essential for human food security and health. However, there is usually disharmony between yield and quality. Seed storage protein (SSP) and starch, the predominant components in cereal grains, determine yield and quality, and their coupled synthesis causes a yield-quality trade-off. Therefore, dissection of the underlying regulatory mechanism facilitates simultaneous improvement of yield and quality. Here, we summarize current findings about the synergistic molecular machinery underpin...
Source: Journal of Integrative Plant Biology - February 27, 2024 Category: Biology Authors: Shuanghe Cao Bingyan Liu Daowen Wang Awais Rasheed Lina Xie Xianchun Xia Zhonghu He Source Type: research

Structural insights into the Oryza sativa cation transporters HKTs in salt tolerance
In this study, we elucidate the structures of OsHKT1;1 and OsHKT2;1, representing two distinct classes of rice HKTs. The dimeric assembled OsHKTs can be structurally divided into four domains. At the dimer interface, a half-helix or a loop in the third domain is coordinated by the C-terminal region of the opposite subunit. Additionally, we present the structures of OsHKT1;5 salt-tolerant and salt-sensitive variants, a key quantitative trait locus associated with salt tolerance. The salt-tolerant variant of OsHKT1;5 exhibits enhanced Na+ transport capability and displays a more flexible conformation. These findings shed lig...
Source: Journal of Integrative Plant Biology - February 27, 2024 Category: Biology Authors: Ran Gao Yutian Jia Xia Xu Peng Fu Jiaqi Zhou Guanghui Yang Source Type: research

Structural insights into the unusual core photocomplex from a triply extremophilic purple bacterium, Halorhodospira halochloris
J Integr Plant Biol. 2024 Feb 27. doi: 10.1111/jipb.13628. Online ahead of print.ABSTRACTHalorhodospira (Hlr.) halochloris is a triply extremophilic phototrophic purple sulfur bacterium, as it is thermophilic, alkaliphilic, and extremely halophilic. The light-harvesting-reaction center (LH1-RC) core complex of this bacterium displays an LH1-Qy transition at 1,016 nm, which is the lowest-energy wavelength absorption among all known phototrophs. Here we report the cryo-EM structure of the LH1-RC at 2.42 Å resolution. The LH1 complex forms a tricyclic ring structure composed of 16 αβγ-polypeptides and one αβ-heterodimer...
Source: Journal of Integrative Plant Biology - February 27, 2024 Category: Biology Authors: Chen-Hui Qi Guang-Lei Wang Fang-Fang Wang Jie Wang Xiang-Ping Wang Mei-Juan Zou Fei Ma Michael T Madigan Yukihiro Kimura Zheng-Yu Wang-Otomo Long-Jiang Yu Source Type: research