Unexpected Find of a Buttonquail (Aves: Charadriiformes: Turnicidae) in the Lower Pleistocene of Crimea
Dokl Biol Sci. 2024 Jan 8. doi: 10.1134/S0012496623600148. Online ahead of print.ABSTRACTButtonquails (family Turnicidae of the order Charadriiformes) are a morphologically specialized group of small, predominantly tropical birds of open landscapes, which is extremely poorly represented in the fossil record. The article describes a fragmentary humerus of a buttonquail from the Lower Pleistocene of the Taurida Cave in central Crimea. This is the first find of the family Turnicidae in Eurasia in a chronological interval from the Pliocene through the Middle Pleistocene. The find highlights the limited nature of available info...
Source: Doklady Biological Sciences - January 8, 2024 Category: Biology Authors: N V Zelenkov Source Type: research

Two Sources of Mesoderm in Brachiopods
Dokl Biol Sci. 2024 Jan 8. doi: 10.1134/S0012496623700837. Online ahead of print.ABSTRACTIn Bilateria, the formation of the coelomic mesoderm occurs in various ways and is of great significance for comparative embryology and phylogeny. Several early ontogenetic stages were studied in the brachiopod Coptothyris grayi by scanning electron microscopy and cytochemistry combined with confocal laser microscopy. Two sources of the mesoderm were observed to form simultaneously from the anterior and posterior walls of the archenteron at the gastrula stage. Both anterior and posterior rudiments form enterocoely as unpaired protrusio...
Source: Doklady Biological Sciences - January 8, 2024 Category: Biology Authors: T V Kuzmina E N Temereva Source Type: research

Efficient Editing of the CXCR4 Locus Using Cas9 Ribonucleoprotein Complexes Stabilized with Polyglutamic Acid
Dokl Biol Sci. 2024 Jan 8. doi: 10.1134/S0012496623700862. Online ahead of print.ABSTRACTGene editing using the CRISPR/Cas9 system provides new opportunities to treat human diseases. Approaches aimed at increasing the efficiency of genome editing are therefore important to develop. To increase the level of editing of the CXCR4 locus, which is a target for gene therapy of HIV infection, the Cas9 protein was modified by introducing additional NLS signals and ribonucleoprotein complexes of Cas9 and guide RNA were stabilized with poly-L-glutamic acid. The approach allowed a 1.8-fold increase in the level of CXCR4 knockout in t...
Source: Doklady Biological Sciences - January 8, 2024 Category: Biology Authors: D S Golubev D S Komkov M V Shepelev D V Mazurov N A Kruglova Source Type: research

Activities of Energy Metabolism Enzymes in Atlantic Salmon Salmo salar L. Smolts and Parr Grown under Different Light Regimens
Dokl Biol Sci. 2024 Jan 8. doi: 10.1134/S0012496623700850. Online ahead of print.ABSTRACTActivities of enzymes of energy and carbohydrate metabolism in muscles and the liver were studied in Atlantic salmon Salmo salar L. smolts and parr grown under continuous or natural lighting and different feeding regimens in autumn followed by a short photoperiod in winter. Enzyme activities were found to differ between test and control salmon groups and between parr and smolts sampled at the end of the winter period. Smolts grown under continuous lighting and round-the-clock feeding differed from other groups by having higher cytochro...
Source: Doklady Biological Sciences - January 8, 2024 Category: Biology Authors: M V Kuznetsova M A Rodin N S Shulgina M Yu Krupnova A E Kuritsin S A Murzina N N Nemova Source Type: research

Collagen Contents of < sup > 13 < /sup > С and < sup > 15 < /sup > N Isotopes in Bones of Small Cave Bear Ursus (Spelaearctos) rossicus Borissiak, 1930 (Mammalia, Carnivora, Ursidae) from Western Siberia
Dokl Biol Sci. 2024 Jan 8. doi: 10.1134/S0012496623700874. Online ahead of print.ABSTRACTThe 13C and 15N isotope contents in collagen were determined in bones of the small cave bear Ursus (Spelaearctos) rossicus Borissiak, 1930 from three regions of Western Siberia. The bones dated back to marine isotope stage (MIS) 3 and belonged to mature males and females. Some of the samples differed only in δ15N. Bears of all samples were from the same trophic level. Trophic shifts were observed between females and males in one region and between males of two regions. The small cave bear of Western Siberia differed from the small cav...
Source: Doklady Biological Sciences - January 8, 2024 Category: Biology Authors: P A Kosintsev Source Type: research