Hydrogels Based on Recombinant Spidroin Stimulate Proliferation and Migration of Human Corneal Cells
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623600173. Online ahead of print.ABSTRACTThe effect of recombinant spidroin (RS) hydrogel (HG) on anterior epithelial cells and keratocytes of the human cornea was studied in vitro. Corneal injuries are highly prevalent in developing countries according to the World Health Organization. Various technologies have recently been proposed to restore the damaged surface of the cornea. Use of biodegradable silk-based materials, including recombinant analogs of the spider silk protein spidroin, is an important avenue of research in the field of wound healing and corneal regeneratio...
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: O I Agapova D S Ostrovsky M Kh Khubetsova T Z Kerimov S A Borzenok V G Bogush L I Davydova S E Cheperegin A E Efimov I I Agapov V G Debabov Source Type: research
The RRE-Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623700886. Online ahead of print.ABSTRACTDelivery of ribonucleoprotein complexes of Cas9 nuclease and guide RNA into target cells with virus-like particles (VLP) is one of the novel methods of genome editing and is suitable for gene therapy of human diseases in the future. The efficiency of genome editing with VLPs depends on the Cas9 packaging into VLPs, the process mediated by the viral Gag protein. To improve the packaging of Cas9 into NanoMEDIC VLPs, plasmid constructs for Cas9 and Gag expression were modified by adding the HIV Rev response element (RRE), which was expec...
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: N A Kruglova D S Komkov D V Mazurov M V Shepelev Source Type: research
Nemertodermatida-Endosymbionts of Deep-Sea Acorn Worms (Hemichordata, Torquaratoridae)
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S001249662360015X. Online ahead of print.ABSTRACTWorm-like endosymbionts were found in the hepatic region of the digestive tract of the deep-sea acorn worm Quatuoralisia malakhovi Ezhova et Lukinykh, 2022 (family Torquaratoridae) from the Bering Sea. The symbionts were assigned to the taxon Nemertodermatida on the basis of histological examination. Torquaratoridae are similar in feeding type to holothuroids, which have also been found to have Xenacoelomorpha endosymbionts.PMID:38472683 | DOI:10.1134/S001249662360015X (Source: Doklady Biological Sciences)
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: O V Ezhova A I Lukinykh V V Malakhov Source Type: research
The Sensory Structures of the Carapace of Male Tantulocarida (Crustacea) Are not Homologous to the Lattice Organs of Thecostraca
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623600161. Online ahead of print.ABSTRACTUltrastructural studies on the sensory apparatus of male Tantulocarida (Crustacea) have been conducted for the first time. Comparative morphological analysis with the specialized sensory structures of Thecostraca, known as lattice organs, has allowed for conclusions about possible homologies and further clarification of the phylogenetic position of Tantulocarida.PMID:38472684 | DOI:10.1134/S0012496623600161 (Source: Doklady Biological Sciences)
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: A S Savchenko G A Kolbasov Source Type: research
Hydrogels Based on Recombinant Spidroin Stimulate Proliferation and Migration of Human Corneal Cells
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623600173. Online ahead of print.ABSTRACTThe effect of recombinant spidroin (RS) hydrogel (HG) on anterior epithelial cells and keratocytes of the human cornea was studied in vitro. Corneal injuries are highly prevalent in developing countries according to the World Health Organization. Various technologies have recently been proposed to restore the damaged surface of the cornea. Use of biodegradable silk-based materials, including recombinant analogs of the spider silk protein spidroin, is an important avenue of research in the field of wound healing and corneal regeneratio...
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: O I Agapova D S Ostrovsky M Kh Khubetsova T Z Kerimov S A Borzenok V G Bogush L I Davydova S E Cheperegin A E Efimov I I Agapov V G Debabov Source Type: research
The RRE-Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623700886. Online ahead of print.ABSTRACTDelivery of ribonucleoprotein complexes of Cas9 nuclease and guide RNA into target cells with virus-like particles (VLP) is one of the novel methods of genome editing and is suitable for gene therapy of human diseases in the future. The efficiency of genome editing with VLPs depends on the Cas9 packaging into VLPs, the process mediated by the viral Gag protein. To improve the packaging of Cas9 into NanoMEDIC VLPs, plasmid constructs for Cas9 and Gag expression were modified by adding the HIV Rev response element (RRE), which was expec...
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: N A Kruglova D S Komkov D V Mazurov M V Shepelev Source Type: research
Nemertodermatida-Endosymbionts of Deep-Sea Acorn Worms (Hemichordata, Torquaratoridae)
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S001249662360015X. Online ahead of print.ABSTRACTWorm-like endosymbionts were found in the hepatic region of the digestive tract of the deep-sea acorn worm Quatuoralisia malakhovi Ezhova et Lukinykh, 2022 (family Torquaratoridae) from the Bering Sea. The symbionts were assigned to the taxon Nemertodermatida on the basis of histological examination. Torquaratoridae are similar in feeding type to holothuroids, which have also been found to have Xenacoelomorpha endosymbionts.PMID:38472683 | DOI:10.1134/S001249662360015X (Source: Doklady Biological Sciences)
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: O V Ezhova A I Lukinykh V V Malakhov Source Type: research
The Sensory Structures of the Carapace of Male Tantulocarida (Crustacea) Are not Homologous to the Lattice Organs of Thecostraca
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623600161. Online ahead of print.ABSTRACTUltrastructural studies on the sensory apparatus of male Tantulocarida (Crustacea) have been conducted for the first time. Comparative morphological analysis with the specialized sensory structures of Thecostraca, known as lattice organs, has allowed for conclusions about possible homologies and further clarification of the phylogenetic position of Tantulocarida.PMID:38472684 | DOI:10.1134/S0012496623600161 (Source: Doklady Biological Sciences)
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: A S Savchenko G A Kolbasov Source Type: research
Hydrogels Based on Recombinant Spidroin Stimulate Proliferation and Migration of Human Corneal Cells
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623600173. Online ahead of print.ABSTRACTThe effect of recombinant spidroin (RS) hydrogel (HG) on anterior epithelial cells and keratocytes of the human cornea was studied in vitro. Corneal injuries are highly prevalent in developing countries according to the World Health Organization. Various technologies have recently been proposed to restore the damaged surface of the cornea. Use of biodegradable silk-based materials, including recombinant analogs of the spider silk protein spidroin, is an important avenue of research in the field of wound healing and corneal regeneratio...
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: O I Agapova D S Ostrovsky M Kh Khubetsova T Z Kerimov S A Borzenok V G Bogush L I Davydova S E Cheperegin A E Efimov I I Agapov V G Debabov Source Type: research
The RRE-Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623700886. Online ahead of print.ABSTRACTDelivery of ribonucleoprotein complexes of Cas9 nuclease and guide RNA into target cells with virus-like particles (VLP) is one of the novel methods of genome editing and is suitable for gene therapy of human diseases in the future. The efficiency of genome editing with VLPs depends on the Cas9 packaging into VLPs, the process mediated by the viral Gag protein. To improve the packaging of Cas9 into NanoMEDIC VLPs, plasmid constructs for Cas9 and Gag expression were modified by adding the HIV Rev response element (RRE), which was expec...
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: N A Kruglova D S Komkov D V Mazurov M V Shepelev Source Type: research
Nemertodermatida-Endosymbionts of Deep-Sea Acorn Worms (Hemichordata, Torquaratoridae)
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S001249662360015X. Online ahead of print.ABSTRACTWorm-like endosymbionts were found in the hepatic region of the digestive tract of the deep-sea acorn worm Quatuoralisia malakhovi Ezhova et Lukinykh, 2022 (family Torquaratoridae) from the Bering Sea. The symbionts were assigned to the taxon Nemertodermatida on the basis of histological examination. Torquaratoridae are similar in feeding type to holothuroids, which have also been found to have Xenacoelomorpha endosymbionts.PMID:38472683 | DOI:10.1134/S001249662360015X (Source: Doklady Biological Sciences)
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: O V Ezhova A I Lukinykh V V Malakhov Source Type: research
The Sensory Structures of the Carapace of Male Tantulocarida (Crustacea) Are not Homologous to the Lattice Organs of Thecostraca
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623600161. Online ahead of print.ABSTRACTUltrastructural studies on the sensory apparatus of male Tantulocarida (Crustacea) have been conducted for the first time. Comparative morphological analysis with the specialized sensory structures of Thecostraca, known as lattice organs, has allowed for conclusions about possible homologies and further clarification of the phylogenetic position of Tantulocarida.PMID:38472684 | DOI:10.1134/S0012496623600161 (Source: Doklady Biological Sciences)
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: A S Savchenko G A Kolbasov Source Type: research
Hydrogels Based on Recombinant Spidroin Stimulate Proliferation and Migration of Human Corneal Cells
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623600173. Online ahead of print.ABSTRACTThe effect of recombinant spidroin (RS) hydrogel (HG) on anterior epithelial cells and keratocytes of the human cornea was studied in vitro. Corneal injuries are highly prevalent in developing countries according to the World Health Organization. Various technologies have recently been proposed to restore the damaged surface of the cornea. Use of biodegradable silk-based materials, including recombinant analogs of the spider silk protein spidroin, is an important avenue of research in the field of wound healing and corneal regeneratio...
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: O I Agapova D S Ostrovsky M Kh Khubetsova T Z Kerimov S A Borzenok V G Bogush L I Davydova S E Cheperegin A E Efimov I I Agapov V G Debabov Source Type: research
The RRE-Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S0012496623700886. Online ahead of print.ABSTRACTDelivery of ribonucleoprotein complexes of Cas9 nuclease and guide RNA into target cells with virus-like particles (VLP) is one of the novel methods of genome editing and is suitable for gene therapy of human diseases in the future. The efficiency of genome editing with VLPs depends on the Cas9 packaging into VLPs, the process mediated by the viral Gag protein. To improve the packaging of Cas9 into NanoMEDIC VLPs, plasmid constructs for Cas9 and Gag expression were modified by adding the HIV Rev response element (RRE), which was expec...
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: N A Kruglova D S Komkov D V Mazurov M V Shepelev Source Type: research
Nemertodermatida-Endosymbionts of Deep-Sea Acorn Worms (Hemichordata, Torquaratoridae)
Dokl Biol Sci. 2024 Mar 12. doi: 10.1134/S001249662360015X. Online ahead of print.ABSTRACTWorm-like endosymbionts were found in the hepatic region of the digestive tract of the deep-sea acorn worm Quatuoralisia malakhovi Ezhova et Lukinykh, 2022 (family Torquaratoridae) from the Bering Sea. The symbionts were assigned to the taxon Nemertodermatida on the basis of histological examination. Torquaratoridae are similar in feeding type to holothuroids, which have also been found to have Xenacoelomorpha endosymbionts.PMID:38472683 | DOI:10.1134/S001249662360015X (Source: Doklady Biological Sciences)
Source: Doklady Biological Sciences - March 13, 2024 Category: Biology Authors: O V Ezhova A I Lukinykh V V Malakhov Source Type: research
