Understanding the molecular mechanisms of bisphenol A action in spermatozoa.
Authors: Rahman MS, Pang MG Abstract Bisphenol A (BPA) is an endocrine-disrupting chemical that is capable of interfering with the normal function of the endocrine system in the body. Exposure to this chemical from BPA-containing materials and the environment is associated with deleterious health effects, including male reproductive abnormalities. A search of the literature demonstrated that BPA, as a toxicant, directly affects the cellular oxidative stress response machinery. Because of its hormone-like properties, it can also bind with specific receptors in target cells. Therefore, the tissue-specific effects of ...
Source: Clinical and Experimental Reproductive Medicine - September 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Investigation of the association of idiopathic male infertility with polymorphisms in the methionine synthase (MTR) gene.
This study aimed to investigate the relationship of the MTR 2756A> G polymorphism with idiopathic male fertility in the Iranian population. Methods: The participants of this study included 100 men with idiopathic infertility and 100 healthy men as the control group. Genotyping of MTR 2756A> G was performed using the polymerase chain reaction and restriction fragment length polymorphism technique. The obtained data were analyzed using SPSS ver. 20.0 with a level of confidence of p G single-nucleotide polymorphism is not a predisposing factor for idiopathic infertility in men. PMID: 31401817 [PubMed - as suppli...
Source: Clinical and Experimental Reproductive Medicine - August 15, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Successful in vitro fertilization pregnancy and delivery after a fertility-sparing laparoscopic operation in a patient with a papillary thyroid carcinoma arising from a mature cystic teratoma.
We describe a case of successful in vitro fertilization pregnancy and delivery after a fertility-sparing laparoscopic operation in a patient with papillary thyroid carcinoma arising from a mature cystic teratoma. PMID: 31401818 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - August 15, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Analysis of vitamin D-binding protein (VDBP) gene polymorphisms in Korean women with and without endometriosis.
Conclusion: Korean women with endometriosis had lower total serum 25(OH)D concentrations than controls. Neither serum VDBP concentrations nor polymorphisms in the gene coding for VDBP were associated with endometriosis. Further studies are needed to investigate the pathophysiology and clinical implications of 25(OH)D and VDBP in endometriosis. PMID: 31405270 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - August 15, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

The impact of two doses of coenzyme Q10 on semen parameters and antioxidant status in men with idiopathic oligoasthenoteratospermia.
Conclusion: CoQ10 supplementation improved semen parameters and antioxidant status in men with idiopathic OAT, with a greater improvement shown in men who took 400 mg/day than in those who took 200 mg/day. PMID: 31408928 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - August 15, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Correlations between embryo morphokinetic development and maternal age: Results from an intracytoplasmic sperm injection program.
Authors: Faramarzi A, Khalili MA, Mangoli E Abstract Objective: It is widely accepted that aging decreases women's fertility capacity. The aim of this study was to assess correlations between maternal age and the morphokinetic parameters and cleavage pattern of embryos. Methods: The morphokinetics of embryos derived from women 40 years of age were compared retrospectively in terms of time of second polar body extrusion, time of pronuclei appearance, time of pronuclei fading, and time of two to eight discrete cells (t2-t8). Furthermore, abnormal cleavage patterns such as uneven blastomeres at the two-cell stage,...
Source: Clinical and Experimental Reproductive Medicine - June 22, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Primordial follicle activation as new treatment for primary ovarian insufficiency.
Authors: Lee HN, Chang EM Abstract Primordial follicle activation is a process in which individual primordial follicles leave their dormant state and enter a growth phase. While existing hormone stimulation strategies targeted the growing follicles, the remaining dormant primordial follicles were ruled out from clinical use. Recently, in vitro activation (IVA), which is a method for controlling primordial follicle activation, has provided an innovative technology for primary ovarian insufficiency (POI) patients. IVA was developed based on Hippo signaling and phosphatase and tensin homolog (PTEN)/phosphatidylinosito...
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Clinical application of serum anti-M ├╝llerian hormone in women.
Clinical application of serum anti-Müllerian hormone in women. Clin Exp Reprod Med. 2019 Jun;46(2):50-59 Authors: Oh SR, Choe SY, Cho YJ Abstract Anti-Müllerian hormone (AMH), a peptide growth factor of the transforming growth factor-β family, is a reliable marker of ovarian reserve. Regarding assisted reproductive technology, AMH has been efficiently used as a marker to predict ovarian response to stimulation. The clinical use of AMH has recently been extended and emphasized. The uses of AMH as a predictive marker of menopause onset, diagnostic tool for polycystic ovary syndrome, and as...
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Evaluation of black glutinous rice (Oryza sativa L) extract as a novel nuclear stain for human sperm head assessment by microscopic examination.
CONCLUSION: The results showed good agreement and a strong correlation between the rapid PAP and black rice extract stains. The advantages of black rice extract as a novel substitute for hematoxylin for nuclear staining include ease of preparation, local availability, and favorable nuclear staining properties. Further studies could also focus on comparing staining techniques in clinical samples. PMID: 31181873 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Does conventional freezing affect sperm DNA fragmentation?
CONCLUSION: Conventional freezing seems to damage some sperm parameters, in particular causing a reduction in sperm DNA integrity. PMID: 31181874 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Differential gene expression analysis of human cumulus cells.
CONCLUSION: Although further confirmatory studies are necessary, findings of this study suggest that these pathways, especially circadian entrainment in cumulus cells, may be essential for embryo development and pregnancy. PMID: 31181875 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Ultrasound guidance versus the blind method for intrauterine catheter insemination: A randomized controlled trial.
CONCLUSION: The conventional blind method for intrauterine catheter insemination is recommended for patients undergoing IUI treatment. The use of ultrasound during the insemination procedure increased the need for trained personnel to perform ultrasonography and increased the cost, but added no extra benefits for patients or clinicians. PMID: 31181876 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Delayed recovery of a patient with obstructive azoospermia and a history of acute epididymitis.
We present a young male patient who had azoospermia and a history of acute epididymitis who experienced delayed recovery, with complete restoration of sperm production and the ability to conceive naturally. PMID: 31181877 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Mesenchymal stem cells for restoration of ovarian function.
Authors: Yoon SY Abstract With the progress of regenerative medicine, mesenchymal stem cells (MSCs) have received attention as a way to restore ovarian function. It has been reported that MSCs derived from bone marrow, adipose, umbilical cord blood, menstrual blood, and amniotic fluid improved ovarian function. In light of previous studies and advances in this field, there are increased expectations regarding the utilization of MSCs to restore ovarian function. This review summarizes recent research into potential applications of MSCs in women with infertility or primary ovarian insufficiency, including cases where...
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation.
Authors: Lee D, Jee BC Abstract OBJECTIVE: Density gradient centrifugation (DGC) is frequently used to isolate high-motility fractions of spermatozoa. We compared the efficacy of four DGC media in terms of the percentage of morphologically normal spermatozoa, DNA fragmentation level, and hyaluronic acid (HA) binding ability. Methods: Thirty men with a total motile spermatozoa count>80 million participated. Semen samples were divided into four aliquots, which were processed using PureSperm, PureCeption, Sidney, and SpermGrad media, respectively. The DNA fragmentation level was measured using the Halosperm ass...
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Evaluation of sperm DNA fragmentation using multiple methods: a comparison of their predictive power for male infertility.
Authors: Javed A, Talkad MS, Ramaiah MK Abstract OBJECTIVE: The usual seminal profile has been customarily used for diagnosing male infertility based on an examination of semen samples. However, sperm DNA fragmentation has also been causally linked to reproductive failure, suggesting that it should be evaluated as part of male infertility assessments. To compare the ability of the five most widely utilized methodologies of measuring DNA fragmentation to predict male infertility and reactive oxygen species by Oxisperm kit assay. Methods: In this case-control study, which received ethical committee approval, the ...
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Effects of paternal age on human embryo development in in vitro fertilization with preimplantation genetic screening.
CONCLUSION: Paternal age did not have a significant impact on euploidy rates when PGS was performed. An additional study with a larger sample size is needed to clarify the effects of advanced paternal age on IVF outcomes. PMID: 30827074 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Does bilateral uterine artery ligation have negative effects on ovarian reserve markers and ovarian artery blood flow in women with postpartum hemorrhage?
CONCLUSION: In this study, we showed that bilateral UAL had no negative effects on ovarian reserve or ovarian blood supply, so this treatment should be used as a fertility preservation technique to avoid hysterectomy in patients experiencing PPH. PMID: 30827075 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

A point of confusion for embryologists in the identification of viable spermatozoa by the eosin-nigrosin test.
We report conflicting results in the identification of viable but immotile spermatozoa between the eosin-nigrosin staining and the laser test, which resulted in confusion for embryologists during assisted reproductive technology (ART). Three patients' semen samples that showed no motile spermatozoa are described in this report. To identify viable spermatozoa, we used both the eosin-nigrosin test and the laser test for each sample, and repeated the semen analysis twice in each patient. Viable but immotile spermatozoa selected by the laser test were used for ICSI. Viable spermatozoa were detected by both the eosin-nigrosin a...
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Acknowledgment to reviewers for 2018.
Authors: Abstract PMID: 30827077 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Bone marrow-derived stem cells contribute to regeneration of the endometrium.
Authors: Lee YJ, Yi KW Abstract Stem cells are undifferentiated cells capable of self-renewal and differentiation into various cell lineages. Stem cells are responsible for the development of organs and regeneration of damaged tissues. The highly regenerative nature of the human endometrium during reproductive age suggests that stem cells play a critical role in endometrial physiology. Bone marrow-derived cells migrate to the uterus and participate in the healing and restoration of functionally or structurally damaged endometrium. This review summarizes recent research into the potential therapeutic effects of bone...
Source: Clinical and Experimental Reproductive Medicine - December 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Sex hormones alter the response of Toll-like receptor 3 to its specific ligand in fallopian tube epithelial cells.
Conclusion: These results imply that sex hormones exerted a suppressive effect on the function of TLR3 in the fallopian tube cell line when different concentrations of sex hormones were present. The current results also suggest that estrogen receptor beta and nuclear progesterone receptor B are likely to mediate the hormonal regulation of TLR3, as these two receptors are the main estrogen and progesterone receptors in OE-E6/E7 cell line. PMID: 30538945 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - December 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Protective effects of saffron against zearalenone-induced alterations in reproductive hormones in female mice (Mus musculus).
Conclusion: The administration of saffron to female mice resulted in a significant reduction in ZEA-induced alterations in reproductive hormone levels, the size of the uterus, and the morphology of the ovaries. PMID: 30538946 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - December 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia.
Conclusion: The testicular pool proved to be easily analyzable, practical, manageable, and more accurate for predicting sperm retrieval than standard testicular biopsy. PMID: 30538947 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - December 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Increased cryo-survival rate in ejaculated human sperm from infertile men following pre-freeze in vitro myo-inositol supplementation.
Authors: Saleh R, Assaf H, Abd El Maged WM, Elsuity M, Fawzy M Abstract Objective: To investigate the effects of in vitro myo-inositol (Myo-Ins) supplementation of cryopreserved human semen on the cryo-survival rate (CSR). Methods: Semen samples were obtained from 41 infertile men. Following routine semen analysis, each sample was divided into two equal aliquots (0.5 mL each). One aliquot was treated with 1 mg of Myo-Ins dissolved in 10 µL of sperm preparation medium. The second aliquot was treated with 10 µL of the same medium (control). Both aliquots were incubated for 20 minutes prior to freezing...
Source: Clinical and Experimental Reproductive Medicine - December 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

A retrospective analysis of the follicle-stimulating hormone starting dose in expected normal responders undergoing their first in vitro fertilization cycle: proposed dose versus empiric dose.
Conclusion: The use of the proposed FSH starting dose determined using La Marca's nomogram did not enhance the optimal ovarian response rate or pregnancy rate in expected normal responders. Individualization of the FSH starting dose by La Marca's nomogram appears to have no distinct advantages over empiric choice of the dose in expected normal responders. PMID: 30538949 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - December 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women.
Authors: Pongsuthirak P, Vutyavanich T Abstract Objective: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. Methods: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later. Mature oocytes ...
Source: Clinical and Experimental Reproductive Medicine - December 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

What should be done for men with sperm DNA fragmentation?
Authors: Kim GY Abstract In an age when a small quantity of sperm can lead to pregnancy through in vitro fertilization or intracytoplasmic sperm injection, selecting healthy sperm is important. Sperm DNA fragmentation (SDF) is known to be higher in infertile men. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) and the alkaline comet test are SDF tests that directly measure DNA damage and have shown closer correlations with assisted reproduction results than indirect tools such as the sperm chromatin structure assay or the sperm chromatic dispersion test. It is diff...
Source: Clinical and Experimental Reproductive Medicine - September 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Rapid freezing versus Cryotop vitrification of mouse two-cell embryos.
Authors: Inna N, Sanmee U, Saeng-Anan U, Piromlertamorn W, Vutyavanich T Abstract Objective: To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. Methods: Two-cell mouse embryos were randomly allocated into three groups: a non-frozen control group (group 1, n=300), a group that underwent Cryotop vitrification (group 2, n=300), and a group that underwent our in-house freezing method (group 3, n=300). Results: There were no significant differences between groups 2 and 3 in the...
Source: Clinical and Experimental Reproductive Medicine - September 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Associations of serum fetuin-A and oxidative stress parameters with polycystic ovary syndrome.
Conclusion: The data obtained from the present study suggest that higher levels of both serum fetuin-A and oxidative stress markers might be related with PCOS. PMID: 30202741 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - September 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Early fragment removal on in vitro fertilization day 2 significantly improves the subsequent development and clinical outcomes of fragmented human embryos.
This study was a retrospective analysis involving 191 IVF cycles in which all embryos had over 10% fragmentation (grade 3 or 4) on day 2 of the IVF-embryo transfer cycle from March 2015 to December 2017. IVF cycles were divided into the fragment removal group (n=87) and the no fragment removal group (n=104) as a control cohort. Before fragment removal, embryos with fragmentation on day 2 were incubated in Ca2+- and Mg2+-free biopsy medium under paraffin oil for 30 minutes. Microsurgical fragment removal was performed with later-assisted hatching and a handmade suction micropipette that had an outer diameter of 30 µm....
Source: Clinical and Experimental Reproductive Medicine - September 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Thinning and drilling laser-assisted hatching in thawed embryo transfer: A randomized controlled trial.
Conclusion: The present study demonstrated that partial ZP thinning or drilling resulted in similar outcomes in implantation and pregnancy rates using thawed embryos, irrespective of women's age or ZP thickness. PMID: 30202743 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - September 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Early gonadotropin-releasing hormone antagonist protocol in women with polycystic ovary syndrome: A preliminary randomized trial.
Conclusion: In PCOS patients undergoing IVF, early administration of a GnRH antagonist may possibly lead to benefits due to a reduced incidence of moderate-to-severe OHSS in high-risk subjects with a better clinical pregnancy rate per embryo transfer. Further studies with more subjects are required. PMID: 30202744 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - September 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Angiopoietin-1 and -2 and vascular endothelial growth factor expression in ovarian grafts after cryopreservation using two methods.
Conclusion: Our results indicate that Angpt-2 may play an important role in ovarian tissue transplantation after cryopreservation although further studies are needed to understand its exact function. PMID: 30202745 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - September 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

The effects of oral antioxidants on the semen of men with idiopathic oligoasthenoteratozoospermia.
Authors: Alahmar AT Abstract It has been estimated that approximately 15% of reproductive-age couples suffer from infertility. Male factors contribute to almost half of infertility cases, and in many patients the underlying cause of oligoasthenoteratozoospermia is unknown. Accumulating evidence suggests that oxidative stress plays a role as a contributing factor to male infertility, and reactive oxygen species have been shown to impair sperm function and motility and to damage sperm membrane and DNA. Therefore, this review explored the evidence provided by studies published from 2002 to 2017 on the impact of oral a...
Source: Clinical and Experimental Reproductive Medicine - July 11, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Current clinical applications of platelet-rich plasma in various gynecological disorders: An appraisal of theory and practice.
Authors: Dawood AS, Salem HA Abstract The purpose of this paper is to review the current clinical uses of platelet-rich plasma (PRP) in the field of gynecology. All relevant articles published from January 2000 to December 2017 were reviewed and analyzed. The articles on PRP in the field of gynecology were mainly case series, pilot studies, or case reports. PRP is currently considered a new therapeutic modality for some disorders that are refractory to conventional drugs. PMID: 29984206 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - July 11, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells.
Conclusion: Human WJ-MSCs can be transdifferentiated into more advanced stages of germ cells by a simple two-step induction protocol using retinoic acid and Sertoli cell-conditioned medium. PMID: 29984207 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - July 11, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Evaluation of prooxidant-antioxidant balance in in vitro fertilization-conceived mice.
Conclusion: Since free radicals contribute to several pathological conditions and antioxidants play an important protective role against oxidative stress, evaluating the oxidant-antioxidant balance is very important. Although the results of this study showed that the quality of the defense mechanism against free radicals was not significantly different between the IVF-conceived and naturally conceived mice, other parameters of metabolic dysfunction need to be measured. PMID: 29984208 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - July 11, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Seminal reactive oxygen species and total antioxidant capacity: Correlations with sperm parameters and impact on male infertility.
Authors: Subramanian V, Ravichandran A, Thiagarajan N, Govindarajan M, Dhandayuthapani S, Suresh S Abstract Objective: The aim of this study was to measure reactive oxygen species (ROS) production and total antioxidant capacity (TAC) in the seminal fluid of the male partners in couples undergoing intrauterine insemination and to evaluate correlations between these values and their semen parameters. Methods: The study was conducted at Vamsam Fertility Center, Coimbatore, India and enrolled 110 male patients from whom semen samples were collected. ROS production was measured by a thiobarbituric acid reactive spec...
Source: Clinical and Experimental Reproductive Medicine - July 11, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

A new biomarker for the early diagnosis of ovarian torsion: SCUBE-1.
Conclusion: We believe that SCUBE-1 may be a promising biomarker for the early diagnosis of OT. PMID: 29984210 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - July 11, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Variable localization of Toll-like receptors in human fallopian tube epithelial cells.
Conclusion: Our findings suggest that TLR expression in the fallopian tubes is cell-type-specific. According to our results, ciliated cells may play more effective role than non-ciliated cells in the innate immune defense of the fallopian tubes, and in interactions with gametes and embryos. PMID: 29662819 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Effects of selenium on the survival and invasion of trophoblasts.
Conclusion: These results will be used as basic data for understanding the mechanism of how trophoblast cells respond to oxidative stress and how selenium promotes the upregulation of related genes and improves the survival rate and invasive ability of trophoblasts through regulating mitochondrial activity. These results suggest that selenium may be used in reproductive medicine for purposes including infertility treatment. PMID: 29662820 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Sperm chromatin and DNA integrity, methyltransferase mRNA levels, and global DNA methylation in oligoasthenoteratozoospermia.
Authors: Rahiminia T, Yazd EF, Fesahat F, Moein MR, Mirjalili AM, Talebi AR Abstract Objective: To investigate sperm chromatin/DNA integrity, global DNA methylation, and DNMT mRNA transcription in men with oligoasthenoteratozoospermia (OAT) compared with normozoospermic men. Methods: Semen samples from 32 OAT patients who comprised the case group and 32 normozoospermic men who comprised the control group were isolated and purified using a standard gradient isolation procedure according to World Health Organization criteria. DNMT1, DNMT3A, and DNMT3B transcripts were then compared between groups using real-time ...
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Effects of three-area laser-assisted zona thinning in 8-cell human embryos on pregnancy outcomes in vitro fertilization.
This study conducted a preliminary examination of the effects of three-area laser-assisted zona thinning (LAZT) during the cleavage stage of embryo development on the hatching process in human in vitro fertilization-embryo transfer (IVF-ET) with subjects of advanced female age or frozen-thawed (FT) embryos. Methods: Eight-cell stage embryos were treated with LAZT in three areas of the zona pellucida at 120° intervals. The control group was embryos without LAZT. Of the 72 consecutive fresh cycles and the 28 FT embryo transfer cycles, the patients in 55 fresh cycles and 17 FT cycles declined LAZT, and those cycles we...
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

The likelihood of achieving pregnancy through timed coitus in young infertile women with decreased ovarian reserve.
Authors: Koo HS, Song IO, Cha SH, Park CW, Kim HO Abstract Objective: To evaluate the pregnancy rate and time to pregnancy after timed coitus with or without superovulation in infertile young women younger than 35 years old with low serum anti-Müllerian hormone (AMH) levels (
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Knowledge regarding factors that influence fertility in Thai reproductive-age population living in urban area: A cross-sectional study.
Conclusion: A considerable knowledge gap about the factors that influence fertility was identified in reproductive-age individuals in an urban area of Thailand. This issue should be urgently addressed by promoting fertility awareness through education, discussions about social perceptions regarding fertility, and reliable sources of knowledge. PMID: 29662824 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Successful onco-testicular sperm extraction from a testicular cancer patient with a single testis and azoospermia.
We report the case of a testicular tumor in the solitary testis of a patient who had previously undergone successful contralateral orchiectomy and whose sperm was preserved by onco-testicular sperm extraction. A 35-year-old patient presented with swelling of his right scrotum that had lasted for 1 month. His medical history included a contralateral orchiectomy during childhood. Ultrasonography revealed a mosaic echoic area in his scrotum, suggesting a testicular tumor. The lesion was palpated within the normal testicular tissue along its edge and semen analysis showed azoospermia. Radical inguinal orchiectomy and onco-test...
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

An infertile patient with Y chromosome b1/b3 deletion presenting with congenital bilateral absence of the vas deferens with normal spermatogenesis.
We report the case of a 46-year-old Chinese male patient who visited our clinic complaining of infertility. Semen analysis revealed azoospermia, and azoospermia factor c region partial deletion (b1/b3) was detected using Y chromosome microdeletion analysis. Testicular sperm extraction was performed after genetic counseling. The bilateral ductus deferens and a portion of the epididymis were absent, whereas the remaining epididymis was expanded. Motile intratesticular spermatozoa were successfully extracted from the seminiferous tubule. On histopathology, nearly complete spermatogenesis was confirmed in almost every seminife...
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Retrospective study of single vitrified-warmed blastocyst transfer cycles according to the presence of morphokinetic variables.
This study retrospectively assessed whether time-lapse data relating to developmental timing and morphology were associated with clinical outcomes, with the eventual goal of using morphokinetic variables to select embryos prospectively for cryopreservation. In this study, we examined the clinical outcomes of single vitrified-warmed blastocyst transfer cycles that were cultured in a time-lapse incubation system. The morphokinetic variables included uneven pronuclei, an uneven blastomere, multinucleation, and direct, rapid, and irregular division. A total of 164 single vitrified-warmed blastocyst transfer cycles were analyze...
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Acknowledgment to reviewers for 2017.
Authors: PMID: 29662828 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - April 19, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research