Advantages of the outgrowth model for evaluating the implantation competence of blastocysts.
Authors: Kim J, Lee J, Jun JH Abstract The implantation process is highly complex and difficult to mimic in vitro, and a reliable experimental model of implantation has yet to be established. Many researchers have used embryo transfer (ET) to assess implantation potential; however, ET with pseudopregnant mice requires expert surgical skills and numerous sacrificial animals. To overcome those economic and ethical problems, several researchers have tried to use outgrowth models to evaluate the implantation potential of embryos. Many previous studies, as well as our experiments, have found significant correlations bet...
Source: Clinical and Experimental Reproductive Medicine - June 11, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Effects of acrylamide in the presence of vitamin E on sperm parameters, chromatin quality, and testosterone levels in mice.
Authors: Anvari M, Talebi AR, Mangoli E, Shahedi A, Ghasemi MR, Pourentezari M Abstract OBJECTIVE: The present study investigated sperm chromatin quality and testosterone levels in acrylamide-treated mice and the possible protective effects of vitamin E on the fertility potential of spermatozoa. METHODS: Thirty-two adult male mice were divided equally into four groups. Group 1 was the control, group 2 received acrylamide (10 mg/kg, water solution), group 3 received vitamin E (100 mg/kg, intraperitoneal), and group 4 received both acrylamide and vitamin E. After 35 days, spermatozoa from the right cauda epididym...
Source: Clinical and Experimental Reproductive Medicine - June 11, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Cumulative live birth rate after up to three consecutive embryo transfer cycles in women with poor ovarian response.
CONCLUSION: Women with ePOR exhibited a lower cumulative LBR than women in the non-ePOR group, and this information should be provided to ePOR women during counseling before starting IVF. PMID: 32521583 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 11, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Intraovarian vascular enhancement via stromal injection of platelet-derived growth factors: Exploring subsequent oocyte chromosomal status and in vitro fertilization outcomes.
Authors: Wood SH, Sills ES Abstract The inverse correlation between maternal age and pregnancy rate represents a major challenge for reproductive endocrinology. The high embryo ploidy error rate in failed in vitro fertilization (IVF) cycles reflects genetic misfires accumulated by older oocytes over time. Despite the application of different follicular recruitment protocols during IVF, gonadotropin modifications are generally futile in addressing such damage. Even when additional oocytes are retrieved, quality is frequently poor. Older oocytes with serious cytoplasmic and/or chromosomal errors are often harvested f...
Source: Clinical and Experimental Reproductive Medicine - May 31, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Endometrial profilin 1: a key player in embryo-endometrial crosstalk.
Conclusion: These observations definitively demonstrate an important role of PFN1 in mediating cell-cell adhesion during the initial stage of embryo implantation and suggest a potential therapeutic target or novel biomarker for patients suffering from implantation failure. PMID: 32466630 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - May 31, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Associations of post-warming embryo or blastocyst development with clinical pregnancy in vitrified embryo or blastocyst transfer cycles.
Conclusion: The top-quality embryo score at transfer and the degree of post-warming embryo development were associated with clinical pregnancy in vitrified cleavage-stage embryo transfer cycles. In vitrified blastocyst transfer cycles, the top-quality blastocyst score at transfer was the only significant factor affecting clinical pregnancy. PMID: 32456411 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - May 29, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Eupatilin treatment inhibits transforming growth factor beta-induced endometrial fibrosis in vitro.
Conclusion: Our findings suggest that suppression of TGF-β-induced signaling by eupatilin might be an effective therapeutic strategy for the treatment of endometrial fibrosis. PMID: 32460455 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - May 29, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Maternal killer-cell immunoglobulin-like receptors and paternal human leukocyte antigen ligands in recurrent pregnancy loss cases in Turkey.
Conclusion: Our data from a Turkish population suggest that male HLA-C2 homozygosity may play an important role in RPL. Additionally, an incidental match between male HLA-C2 and female HLA-C1 ligand KIR receptors might perturb the balance between activatory and inhibitory KIR-ligand interactions during pregnancy in couples affected by RPL. The roles of orphan KIR2DL5 and orphan KIR2DS3 in RPL remain obscure. PMID: 32450627 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - May 26, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

The effect of insemination methods on in vitro maturation outcomes.
Conclusion: There was a comparable yield of in vitro matured oocytes derived from PCOS patients in terms of fertilization, blastocyst formation, implantation rate, and clinical pregnancy between IVF and ICSI insemination. These findings provide valuable insights for choosing assisted reproductive treatment in women with PCOS, as IVM offers promising outcomes and is less invasive and less costly. PMID: 32403207 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - May 15, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Effect of luteal phase support with vaginal progesterone on pregnancy outcomes in natural frozen embryo transfer cycles: A meta-analysis.
Conclusion: The results of this meta-analysis of the currently available literature suggest that LPS with vaginal progesterone in natural FET cycles does not improve the CPR. PMID: 32316709 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - April 24, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Corrigendum to "Management of endometriosis-related infertility: Considerations and treatment options".
Corrigendum to "Management of endometriosis-related infertility: Considerations and treatment options". Clin Exp Reprod Med. 2020 Apr 08;: Authors: Lee D, Kim SK, Lee JR, Jee BC PMID: 32259915 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - April 9, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Effect of thymectomy on the female reproductive cycle in neonatal guinea pigs.
CONCLUSION: The results of this study underscore the importance of the thymus in the neonatal period for normal female reproductive function. PMID: 32146772 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 10, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

The effects of Commiphora mukul extract on spermatogenesis and testosterone levels in male diabetic rats.
Authors: Rezaei AA, Salehi I, Karimi SA, Rahnama M Abstract OBJECTIVE: The strong antioxidant activity of Commiphora mukul prompted us to conduct the present study to explore whether treatment with C. mukul extract (CME) would have any protective influence on sperm parameters, testosterone levels, and plasma glucose levels in streptozotocin (STZ)-induced diabetic rats. METHODS: Male Wistar rats were randomly divided into four groups: control, control animals treated with CME, diabetic animals, and diabetic animals treated with CME. CME extract (300 mg/kg) was administered for 60 days by daily gavage. Diabetes w...
Source: Clinical and Experimental Reproductive Medicine - March 10, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Identification of microRNAs and their target genes in the placenta as biomarkers of inflammation.
CONCLUSION: These results provide candidate miRNAs and their target genes that could be used as placental biomarkers of inflammation. These candidates may be useful for further miRNA-based biomarker development. PMID: 32146774 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 10, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Upregulation of the RNF8 gene can predict the presence of sperm in azoospermic individuals.
CONCLUSION: This study points out a significant association between the expression of RNF8 and the presence of sperm in NOA patients, which suggests that quantified RNF8 expression in testicular biopsy samples may be a valuable biomarker for predicting the presence of spermatozoa in biopsy samples. PMID: 32146775 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 10, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

The effect of vitamin C on the gene expression profile of sperm protamines in the male partners of couples with recurrent pregnancy loss: A randomized clinical trial.
CONCLUSION: Daily oral administration of vitamin C may improve human sperm parameters and DNA integrity by increasing protamine gene expression levels in the male partners of couples with RPL. The beneficial effects of vitamin C supplementation as an antioxidant for the male partners of couples with RPL could lead to improved pregnancy outcomes in these cases. PMID: 32146776 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 10, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Impact of irregular marital cohabitation on quality of life and sexual dysfunction in infertile men from upper Egypt.
CONCLUSION: Irregular patterns of marital cohabitation had an adverse effect on quality of life and sexual function in infertile men. PMID: 32146777 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 10, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Acknowledgment to reviewers for 2019.
Authors: PMID: 32146778 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 10, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Management of endometriosis-related infertility: Considerations and treatment options.
Authors: Lee D, Kim SK, Lee JR, Jee BC Abstract Endometriosis is a common inflammatory disease in women of reproductive age and is one of the major causes of infertility. Endometriosis causes a sustained reduction of ovarian reserve through both physical mechanisms and inflammatory reactions, which result in the production of reactive oxygen species and tissue fibrosis. The severity of endometriosis is related to ovarian reserve. With regard to infertility treatment, medical therapy as a neoadjuvant or adjuvant to surgical therapy has no definite beneficial effect. Surgical treatment of endometriosis can lead to ov...
Source: Clinical and Experimental Reproductive Medicine - February 25, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Nitric oxide, 8-hydroxydeoxyguanosine, and total antioxidant capacity in human seminal plasma of infertile men and their relationship with sperm parameters.
This study aimed to investigate the levels of nitric oxide (NO), 8-hydroxydesoxyguanosine (8-OHdG), and total antioxidant capacity (TAC) oxidative biomarkers in seminal plasma and their relationship with sperm parameters. Methods: A total of 77 volunteers participated in the study, including fertile (n=40) and infertile men (n=37). NO, 8-OHdG, and TAC levels were measured using the ferric reducing ability of plasma, Griess reagent method and an enzyme-linked immunosorbent assay kit, respectively. Results: The mean values of sperm parameters in the infertile group were significantly lower than those in the fertile group (p
Source: Clinical and Experimental Reproductive Medicine - February 22, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Comparison of male reproductive parameters in mice with type 1 and type 2 diabetes.
Conclusion: T1DM adversely affected reproductive parameters to a greater extent than T2DM. PMID: 32036645 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - February 12, 2020 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Survival of isolated human preantral follicles after vitrification: Analyses of morphology and Fas ligand and caspase-3 mRNA expression.
CONCLUSION: Vitrification did not affect preantral follicle morphology or mRNA expression of the apoptosis markers FasL and caspase-3. Further studies are required to establish whether vitrification affects the outcomes of in vitro culture and the maturation of preantral follicles. PMID: 31813207 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - December 10, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Growth differentiation factor 9 and cumulus cell supplementation in in vitro maturation culture media enhances the viability of human blastocysts.
Authors: Chatroudi MH, Khalili MA, Ashourzadeh S, Anbari F, Shahedi A, Safari S Abstract OBJECTIVE: In vitro maturation (IVM) of immature oocytes can be useful for some infertile patients. In IVM programs, the rates of embryo formation and pregnancy are low. Therefore, it is essential to recognize the main factors involved in regulating oocyte maturation in vitro. The purpose of this study was to investigate the effects of growth differentiation factor 9 (GDF9) and cumulus cell (CC) supplementation in IVM medium on the rates of embryo formation and viability of human blastocysts. METHODS: A total of 80 germinal...
Source: Clinical and Experimental Reproductive Medicine - December 10, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system.
CONCLUSION: Our results showed that a dynamic oxygen concentration (decreasing from 5% to 2%) had beneficial effects on mouse pre- and peri-implantation development. Optimized, dynamic changing of oxygen concentrations using the novel DCGS incubator could improve the developmental competence of in vitro cultured embryos in a human in vitro fertilization and embryo transfer program. PMID: 31813209 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - December 10, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Birth of a healthy baby after preimplantation genetic diagnosis in a carrier of mucopolysaccharidosis type II: The first case in Korea.
Authors: Ko DS, Lee SH, Park CW, Lim CK Abstract Mucopolysaccharidosis type II (MPS II) is a rare X-linked recessive lysosomal storage disease caused by mutation of the iduronate-2-sulfatase gene. The mutation results in iduronate-2-sulfatase deficiency, which causes the progressive accumulation of heparan sulfate and dermatan sulfate in cellular lysosomes. The phenotype, age of onset, and symptoms of MPS II vary; accordingly, the disease can be classified into either the early-onset type or the late-onset type, depending on the age of onset and the severity of the symptoms. In patients with severe MPS II, symptoms...
Source: Clinical and Experimental Reproductive Medicine - December 10, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Corrigendum to "Evaluation of sperm DNA fragmentation using multiple methods: a comparison of their predictive power for male infertility".
Corrigendum to "Evaluation of sperm DNA fragmentation using multiple methods: a comparison of their predictive power for male infertility". Clin Exp Reprod Med. 2019 12;46(4):211 Authors: Javed A, Talkad MS, Ramaiah MK PMID: 31813211 [PubMed - in process] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - December 10, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Specific tail swelling pattern in hypo-osmotic solution as a predictor of DNA fragmentation status in human spermatozoa.
Conclusion: We found that the type "d" spermatozoa displayed no DNA fragmentation, but the other types of spermatozoa also displayed very low rates of DNA fragmentation. This result may be associated with the processing of the spermatozoa by density gradient centrifugation and the swim-up technique. PMID: 31739655 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - November 20, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Inter-ovarian differences in ultrasound markers of ovarian size in women with polycystic ovary syndrome.
Conclusion: Ovarian size is different between the right and the left ovary in women with polycystic ovary syndrome. PMID: 31739656 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - November 20, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Macroprolactinoma in a young man presenting with erectile dysfunction.
Authors: Song SH, Lee J, Kim DS Abstract Hyperprolactinemia due to a pituitary adenoma is a rare cause of erectile dysfunction (ED). The prevalence of clinically apparent prolactinomas is reported to be from 6-10 to 50 per 100,000. A few reports have been published of prolactinoma presenting with ED. Here, we report a rare case of a young man who presented with ED as a chief complaint and who was diagnosed with a huge prolactinoma, and we discuss a related fertility issue. PMID: 31739657 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - November 20, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Misuse of testosterone replacement therapy in men in infertile couples and its influence on infertility treatment.
This study examined the records of 20 consecutive male patients diagnosed with azoospermia or severe oligozoospermia (
Source: Clinical and Experimental Reproductive Medicine - November 20, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Live birth outcomes of vitrified embryos generated under growth hormone stimulation are improved for women categorized as poor-prognosis.
Authors: Keane KN, Ye Y, Hinchliffe PM, Regan SL, Dhaliwal SS, Yovich JL Abstract Objective: To determine the clinical pregnancy (CP) and live birth (LB) rates arising from frozen embryo transfers (FETs) that had been generated under the influence of in vitro fertilization (IVF) adjuvants given to women categorized as poor-prognosis. Methods: A registered, single-center, retrospective study. A total of 1,119 patients with first FETs cycle include 310 patients with poor prognosis (109 treated with growth hormone [GH], (+)GH group vs. 201 treated with dehydroepiandrosterone, (-)GH group) and 809 patients with goo...
Source: Clinical and Experimental Reproductive Medicine - November 20, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Understanding the molecular mechanisms of bisphenol A action in spermatozoa.
Authors: Rahman MS, Pang MG Abstract Bisphenol A (BPA) is an endocrine-disrupting chemical that is capable of interfering with the normal function of the endocrine system in the body. Exposure to this chemical from BPA-containing materials and the environment is associated with deleterious health effects, including male reproductive abnormalities. A search of the literature demonstrated that BPA, as a toxicant, directly affects the cellular oxidative stress response machinery. Because of its hormone-like properties, it can also bind with specific receptors in target cells. Therefore, the tissue-specific effects of ...
Source: Clinical and Experimental Reproductive Medicine - September 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Investigation of the association of idiopathic male infertility with polymorphisms in the methionine synthase (MTR) gene.
This study aimed to investigate the relationship of the MTR 2756A> G polymorphism with idiopathic male fertility in the Iranian population. Methods: The participants of this study included 100 men with idiopathic infertility and 100 healthy men as the control group. Genotyping of MTR 2756A> G was performed using the polymerase chain reaction and restriction fragment length polymorphism technique. The obtained data were analyzed using SPSS ver. 20.0 with a level of confidence of p G single-nucleotide polymorphism is not a predisposing factor for idiopathic infertility in men. PMID: 31401817 [PubMed - as suppli...
Source: Clinical and Experimental Reproductive Medicine - August 15, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Successful in vitro fertilization pregnancy and delivery after a fertility-sparing laparoscopic operation in a patient with a papillary thyroid carcinoma arising from a mature cystic teratoma.
We describe a case of successful in vitro fertilization pregnancy and delivery after a fertility-sparing laparoscopic operation in a patient with papillary thyroid carcinoma arising from a mature cystic teratoma. PMID: 31401818 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - August 15, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Analysis of vitamin D-binding protein (VDBP) gene polymorphisms in Korean women with and without endometriosis.
Conclusion: Korean women with endometriosis had lower total serum 25(OH)D concentrations than controls. Neither serum VDBP concentrations nor polymorphisms in the gene coding for VDBP were associated with endometriosis. Further studies are needed to investigate the pathophysiology and clinical implications of 25(OH)D and VDBP in endometriosis. PMID: 31405270 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - August 15, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

The impact of two doses of coenzyme Q10 on semen parameters and antioxidant status in men with idiopathic oligoasthenoteratospermia.
Conclusion: CoQ10 supplementation improved semen parameters and antioxidant status in men with idiopathic OAT, with a greater improvement shown in men who took 400 mg/day than in those who took 200 mg/day. PMID: 31408928 [PubMed - as supplied by publisher] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - August 15, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Correlations between embryo morphokinetic development and maternal age: Results from an intracytoplasmic sperm injection program.
Authors: Faramarzi A, Khalili MA, Mangoli E Abstract Objective: It is widely accepted that aging decreases women's fertility capacity. The aim of this study was to assess correlations between maternal age and the morphokinetic parameters and cleavage pattern of embryos. Methods: The morphokinetics of embryos derived from women 40 years of age were compared retrospectively in terms of time of second polar body extrusion, time of pronuclei appearance, time of pronuclei fading, and time of two to eight discrete cells (t2-t8). Furthermore, abnormal cleavage patterns such as uneven blastomeres at the two-cell stage,...
Source: Clinical and Experimental Reproductive Medicine - June 22, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Primordial follicle activation as new treatment for primary ovarian insufficiency.
Authors: Lee HN, Chang EM Abstract Primordial follicle activation is a process in which individual primordial follicles leave their dormant state and enter a growth phase. While existing hormone stimulation strategies targeted the growing follicles, the remaining dormant primordial follicles were ruled out from clinical use. Recently, in vitro activation (IVA), which is a method for controlling primordial follicle activation, has provided an innovative technology for primary ovarian insufficiency (POI) patients. IVA was developed based on Hippo signaling and phosphatase and tensin homolog (PTEN)/phosphatidylinosito...
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Clinical application of serum anti-M ├╝llerian hormone in women.
Clinical application of serum anti-Müllerian hormone in women. Clin Exp Reprod Med. 2019 Jun;46(2):50-59 Authors: Oh SR, Choe SY, Cho YJ Abstract Anti-Müllerian hormone (AMH), a peptide growth factor of the transforming growth factor-β family, is a reliable marker of ovarian reserve. Regarding assisted reproductive technology, AMH has been efficiently used as a marker to predict ovarian response to stimulation. The clinical use of AMH has recently been extended and emphasized. The uses of AMH as a predictive marker of menopause onset, diagnostic tool for polycystic ovary syndrome, and as...
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Evaluation of black glutinous rice (Oryza sativa L) extract as a novel nuclear stain for human sperm head assessment by microscopic examination.
CONCLUSION: The results showed good agreement and a strong correlation between the rapid PAP and black rice extract stains. The advantages of black rice extract as a novel substitute for hematoxylin for nuclear staining include ease of preparation, local availability, and favorable nuclear staining properties. Further studies could also focus on comparing staining techniques in clinical samples. PMID: 31181873 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Does conventional freezing affect sperm DNA fragmentation?
CONCLUSION: Conventional freezing seems to damage some sperm parameters, in particular causing a reduction in sperm DNA integrity. PMID: 31181874 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Differential gene expression analysis of human cumulus cells.
CONCLUSION: Although further confirmatory studies are necessary, findings of this study suggest that these pathways, especially circadian entrainment in cumulus cells, may be essential for embryo development and pregnancy. PMID: 31181875 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Ultrasound guidance versus the blind method for intrauterine catheter insemination: A randomized controlled trial.
CONCLUSION: The conventional blind method for intrauterine catheter insemination is recommended for patients undergoing IUI treatment. The use of ultrasound during the insemination procedure increased the need for trained personnel to perform ultrasonography and increased the cost, but added no extra benefits for patients or clinicians. PMID: 31181876 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Delayed recovery of a patient with obstructive azoospermia and a history of acute epididymitis.
We present a young male patient who had azoospermia and a history of acute epididymitis who experienced delayed recovery, with complete restoration of sperm production and the ability to conceive naturally. PMID: 31181877 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - June 12, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Mesenchymal stem cells for restoration of ovarian function.
Authors: Yoon SY Abstract With the progress of regenerative medicine, mesenchymal stem cells (MSCs) have received attention as a way to restore ovarian function. It has been reported that MSCs derived from bone marrow, adipose, umbilical cord blood, menstrual blood, and amniotic fluid improved ovarian function. In light of previous studies and advances in this field, there are increased expectations regarding the utilization of MSCs to restore ovarian function. This review summarizes recent research into potential applications of MSCs in women with infertility or primary ovarian insufficiency, including cases where...
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation.
Authors: Lee D, Jee BC Abstract OBJECTIVE: Density gradient centrifugation (DGC) is frequently used to isolate high-motility fractions of spermatozoa. We compared the efficacy of four DGC media in terms of the percentage of morphologically normal spermatozoa, DNA fragmentation level, and hyaluronic acid (HA) binding ability. Methods: Thirty men with a total motile spermatozoa count>80 million participated. Semen samples were divided into four aliquots, which were processed using PureSperm, PureCeption, Sidney, and SpermGrad media, respectively. The DNA fragmentation level was measured using the Halosperm ass...
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Evaluation of sperm DNA fragmentation using multiple methods: a comparison of their predictive power for male infertility.
Authors: Javed A, Talkad MS, Ramaiah MK Abstract OBJECTIVE: The usual seminal profile has been customarily used for diagnosing male infertility based on an examination of semen samples. However, sperm DNA fragmentation has also been causally linked to reproductive failure, suggesting that it should be evaluated as part of male infertility assessments. To compare the ability of the five most widely utilized methodologies of measuring DNA fragmentation to predict male infertility and reactive oxygen species by Oxisperm kit assay. Methods: In this case-control study, which received ethical committee approval, the ...
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Effects of paternal age on human embryo development in in vitro fertilization with preimplantation genetic screening.
CONCLUSION: Paternal age did not have a significant impact on euploidy rates when PGS was performed. An additional study with a larger sample size is needed to clarify the effects of advanced paternal age on IVF outcomes. PMID: 30827074 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Does bilateral uterine artery ligation have negative effects on ovarian reserve markers and ovarian artery blood flow in women with postpartum hemorrhage?
CONCLUSION: In this study, we showed that bilateral UAL had no negative effects on ovarian reserve or ovarian blood supply, so this treatment should be used as a fertility preservation technique to avoid hysterectomy in patients experiencing PPH. PMID: 30827075 [PubMed] (Source: Clinical and Experimental Reproductive Medicine)
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

A point of confusion for embryologists in the identification of viable spermatozoa by the eosin-nigrosin test.
We report conflicting results in the identification of viable but immotile spermatozoa between the eosin-nigrosin staining and the laser test, which resulted in confusion for embryologists during assisted reproductive technology (ART). Three patients' semen samples that showed no motile spermatozoa are described in this report. To identify viable spermatozoa, we used both the eosin-nigrosin test and the laser test for each sample, and repeated the semen analysis twice in each patient. Viable but immotile spermatozoa selected by the laser test were used for ICSI. Viable spermatozoa were detected by both the eosin-nigrosin a...
Source: Clinical and Experimental Reproductive Medicine - March 6, 2019 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research