LncRNA LncHrt preserves cardiac metabolic homeostasis and heart function by modulating the LKB1-AMPK signaling pathway
AbstractMetabolic modulation is a promising therapeutic approach to prevent adverse remodeling of the ischemic heart. Because little is known about the involvement of long non-coding RNAs (lncRNAs) in regulating cardiac metabolism, we used unbiased transcriptome profiling in a mouse model of myocardial infarction (MI). We identified a novel cardiomyocyte-enriched lncRNA, calledLncHrt, which regulates metabolism and the pathophysiological processes that lead to heart failure. AAV-basedLncHrt overexpression protects the heart from MI as demonstrated by improved contractile function, preserved metabolic homeostasis, and atten...
Source: Basic Research in Cardiology - August 11, 2021 Category: Cardiology Source Type: research
Atg5 knockdown induces age-dependent cardiomyopathy which can be rescued by repeated remote ischemic conditioning
AbstractAltered autophagy is implicated in several human cardiovascular diseases. Remote ischemic conditioning (RIC) is cardioprotective in multiple cardiovascular injury models and modifies autophagy signaling, but its effect in cardiomyopathy induced by gene manipulation has not been reported. To investigate the cardiac effects of chronically reduced autophagy as a result ofAtg5 knockdown and assess whether RIC can rescue the phenotype.Atg5 knockdown was induced with tamoxifen for 14 days in cardiac-specific conditionalAtg5 flox mice. Autophagy proteins and cardiac function were evaluated by Western blot and echocardio...
Source: Basic Research in Cardiology - July 28, 2021 Category: Cardiology Source Type: research
Regulatory B cells improve ventricular remodeling after myocardial infarction by modulating monocyte migration
AbstractOveractivated inflammatory responses contribute to adverse ventricular remodeling after myocardial infarction (MI). Regulatory B cells (Bregs) are a newly discovered subset of B cells with immunomodulatory roles in many immune and inflammation-related diseases. Our study aims to determine whether the expansion of Bregs exerts a beneficial effect on ventricular remodeling and explore the mechanisms involved. Here, we showed that adoptive transfer of Bregs ameliorated ventricular remodeling in a murine MI model, as demonstrated by improved cardiac function, decreased scar size and attenuated interstitial fibrosis wit...
Source: Basic Research in Cardiology - July 24, 2021 Category: Cardiology Source Type: research
Intravenous metoprolol during ongoing STEMI ameliorates markers of ischemic injury: a METOCARD-CNIC trial electrocardiographic study
AbstractBesides its protective effect against neutrophil-mediated injury at reperfusion, intravenous (IV) metoprolol was recently shown to reduce the progression of ischemic injury in a pig model of ST-segment elevation myocardial infarction (STEMI). Here, we tested the hypothesis that IV metoprolol administration in humans with ongoing STEMI blunts the time ‑dependent progression of ischemic injury assessed by serial electrocardiogram (ECG) evaluations before reperfusion. The METOCARD-CNIC trial randomized 270 anterior STEMI patients to IV metoprolol or control before reperfusion by percutaneous coronary intervention (P...
Source: Basic Research in Cardiology - July 19, 2021 Category: Cardiology Source Type: research
Thiol-based redox-active proteins as cardioprotective therapeutic agents in cardiovascular diseases
AbstractThiol-based redox compounds, namely thioredoxins (Trxs), glutaredoxins (Grxs) and peroxiredoxins (Prxs), stand as a pivotal group of proteins involved in antioxidant processes and redox signaling. Glutaredoxins (Grxs) are considered as one of the major families of proteins involved in redox regulation by removal of S-glutathionylation and thereby reactivation of other enzymes with thiol-dependent activity. Grxs are also coupled to Trxs and Prxs recycling and thereby indirectly contribute to reactive oxygen species (ROS) detoxification. Peroxiredoxins (Prxs) are a ubiquitous family of peroxidases, which play an esse...
Source: Basic Research in Cardiology - July 17, 2021 Category: Cardiology Source Type: research
Increased susceptibility of human endothelial cells to infections by SARS-CoV-2 variants
AbstractCoronavirus disease 2019 (COVID-19) spawned a global health crisis in late 2019 and is caused by the novel coronavirus SARS-CoV-2. SARS-CoV-2 infection can lead to elevated markers of endothelial dysfunction associated with higher risk of mortality. It is unclear whether endothelial dysfunction is caused by direct infection of endothelial cells or is mainly secondary to inflammation. Here, we investigate whether different types of endothelial cells are susceptible to SARS-CoV-2. Human endothelial cells from different vascular beds including umbilical vein endothelial cells, coronary artery endothelial cells (HCAEC)...
Source: Basic Research in Cardiology - July 5, 2021 Category: Cardiology Source Type: research
What the HEC happens around the heart during COVID-19?
(Source: Basic Research in Cardiology)
Source: Basic Research in Cardiology - July 5, 2021 Category: Cardiology Source Type: research
Increased susceptibility of human endothelial cells to infections by SARS-CoV-2 variants
AbstractCoronavirus disease 2019 (COVID-19) spawned a global health crisis in late 2019 and is caused by the novel coronavirus SARS-CoV-2. SARS-CoV-2 infection can lead to elevated markers of endothelial dysfunction associated with higher risk of mortality. It is unclear whether endothelial dysfunction is caused by direct infection of endothelial cells or is mainly secondary to inflammation. Here, we investigate whether different types of endothelial cells are susceptible to SARS-CoV-2. Human endothelial cells from different vascular beds including umbilical vein endothelial cells, coronary artery endothelial cells (HCAEC)...
Source: Basic Research in Cardiology - July 5, 2021 Category: Cardiology Source Type: research
What the HEC happens around the heart during COVID-19?
(Source: Basic Research in Cardiology)
Source: Basic Research in Cardiology - July 5, 2021 Category: Cardiology Source Type: research
