Chemical and mechanical activation of resident cardiac macrophages in the living myocardial slice ex vivo model
AbstractResident cardiac macrophages (rcMACs) are among the most abundant immune cells in the heart. Plasticity and activation are hallmarks of rcMACs in response to changes in the microenvironment, which is essential for in vitro experimentation. The in vivo investigation is confounded by the infiltration of other cells hindering direct studies of rcMACs. As a tool to investigate rcMACs, we applied the ex vivo model of living myocardial slices (LMS). LMS are ultrathin ex vivo multicellular cardiac preparations in which the circulatory network is interrupted. The absence of infiltration in this model enables the investigat...
Source: Basic Research in Cardiology - November 30, 2022 Category: Cardiology Source Type: research
Beta-3 adrenergic receptor overexpression reverses aortic stenosis –induced heart failure and restores balanced mitochondrial dynamics
In conclusion, our results step out that β3AR overexpression might have translational potential as a therapeutic strategy in AS–induced HF. (Source: Basic Research in Cardiology)
Source: Basic Research in Cardiology - November 29, 2022 Category: Cardiology Source Type: research
Elevated platelet –leukocyte complexes are associated with, but dispensable for myocardial ischemia–reperfusion injury
ConclusionWe conclude that myocardial infarction associated sterile inflammation triggers PLC formation, reminiscent of conserved immunothrombotic responses, but without PLC influencing myocardial ischemia and reperfusion injury in return. Our experimental data do not support a therapeutic concept of selectively targeting PLC formation in myocardial infarction. (Source: Basic Research in Cardiology)
Source: Basic Research in Cardiology - November 16, 2022 Category: Cardiology Source Type: research
InsP3R –RyR Ca2+ channel crosstalk facilitates arrhythmias in the failing human ventricle
AbstractDysregulated intracellular Ca2+ handling involving altered Ca2+ release from intracellular stores via RyR channels underlies both arrhythmias and reduced function in heart failure (HF). Mechanisms linking RyR dysregulation and disease are not fully established. Studies in animals support a role for InsP3 receptor Ca2+ channels (InsP3R) in pathological alterations in cardiomyocyte Ca2+ handling but whether these findings translate to the divergent physiology of human cardiomyocytes during heart failure is not determined. Using electrophysiological and Ca2+ recordings in human ventricular cardiomyocytes, we uncovered...
Source: Basic Research in Cardiology - November 15, 2022 Category: Cardiology Source Type: research
Retraction Note to: Cardiomyocyte-specific deletion of endothelin receptor A rescues aging-associated cardiac hypertrophy and contractile dysfunction: role of autophagy
(Source: Basic Research in Cardiology)
Source: Basic Research in Cardiology - November 15, 2022 Category: Cardiology Source Type: research
InsP3R –RyR Ca2+ channel crosstalk facilitates arrhythmias in the failing human ventricle
AbstractDysregulated intracellular Ca2+ handling involving altered Ca2+ release from intracellular stores via RyR channels underlies both arrhythmias and reduced function in heart failure (HF). Mechanisms linking RyR dysregulation and disease are not fully established. Studies in animals support a role for InsP3 receptor Ca2+ channels (InsP3R) in pathological alterations in cardiomyocyte Ca2+ handling but whether these findings translate to the divergent physiology of human cardiomyocytes during heart failure is not determined. Using electrophysiological and Ca2+ recordings in human ventricular cardiomyocytes, we uncovered...
Source: Basic Research in Cardiology - November 15, 2022 Category: Cardiology Source Type: research
Retraction Note to: Cardiomyocyte-specific deletion of endothelin receptor A rescues aging-associated cardiac hypertrophy and contractile dysfunction: role of autophagy
(Source: Basic Research in Cardiology)
Source: Basic Research in Cardiology - November 15, 2022 Category: Cardiology Source Type: research
New insights into cardioprotection, gained by adopting the CAESAR standards of rigor
(Source: Basic Research in Cardiology)
Source: Basic Research in Cardiology - November 11, 2022 Category: Cardiology Source Type: research
Spinal cord astrocytes regulate myocardial ischemia –reperfusion injury
AbstractAstrocytes play a key role in the response to injury and noxious stimuli, but its role in myocardial ischemia –reperfusion (I/R) injury remains largely unknown. Here we determined whether manipulation of spinal astrocyte activity affected myocardial I/R injury and the underlying mechanisms. By ligating the left coronary artery to establish an in vivo I/R rat model, we observed a 1.7-fold rise in glial fib rillary acidic protein (GFAP) protein level in spinal cord following myocardial I/R injury. Inhibition of spinal astrocytes by intrathecal injection of fluoro-citrate, an astrocyte inhibitor, decreased GFAP immu...
Source: Basic Research in Cardiology - November 11, 2022 Category: Cardiology Source Type: research
Non-responsiveness to cardioprotection by ischaemic preconditioning in Ossabaw minipigs with genetic predisposition to, but without the phenotype of the metabolic syndrome
AbstractThe translation of successful preclinical and clinical proof-of-concept studies on cardioprotection to the benefit of patients with reperfused acute myocardial infarction has been difficult so far. This difficulty has been attributed to confounders which patients with myocardial infarction typically have but experimental animals usually not have. The metabolic syndrome is a typical confounder. We hypothesised that there may also be a genuine non-responsiveness to cardioprotection and used Ossabaw minipigs which have the genetic predisposition to develop a diet-induced metabolic syndrome, but before they had develop...
Source: Basic Research in Cardiology - November 11, 2022 Category: Cardiology Source Type: research
New insights into cardioprotection, gained by adopting the CAESAR standards of rigor
(Source: Basic Research in Cardiology)
Source: Basic Research in Cardiology - November 11, 2022 Category: Cardiology Source Type: research
Spinal cord astrocytes regulate myocardial ischemia –reperfusion injury
AbstractAstrocytes play a key role in the response to injury and noxious stimuli, but its role in myocardial ischemia –reperfusion (I/R) injury remains largely unknown. Here we determined whether manipulation of spinal astrocyte activity affected myocardial I/R injury and the underlying mechanisms. By ligating the left coronary artery to establish an in vivo I/R rat model, we observed a 1.7-fold rise in glial fib rillary acidic protein (GFAP) protein level in spinal cord following myocardial I/R injury. Inhibition of spinal astrocytes by intrathecal injection of fluoro-citrate, an astrocyte inhibitor, decreased GFAP immu...
Source: Basic Research in Cardiology - November 11, 2022 Category: Cardiology Source Type: research
