Prevalence of airborne fungal spores in two potato warehouses with different storage conditions
This study constitutes an approach to detecting fungal spores present inside potato warehouses and the influence of the environmental conditions on the prevalence of the different types of fungi. The most common spores wereCladosporium andAspergillus/Penicillium representing more than 94% of the identified spores of fungi. However, some potential fungal potato pathogens such asAlternaria,Helminthosporium,Fusarium andPhytophthora were also detected. In the warehouse without control of the storage conditions, the most represented fungal spores were fromCladosporium followed byAspergillus/Penicillium, Helminthosporium andAlte...
Source: Aerobiologia - February 6, 2021 Category: Environmental Health Source Type: research
Microbial community structure in PM 2.5 affected by burning fireworks during the 2019 Spring Festival in Guilin, a touristic city in South China
In this study, PM2.5 samples were collected at four different sites in the city of Guilin during Spring Festival from February 4 to 8, 2019 and a high-throughput sequencing technology was used to investigate the variations of bacterial diversity and community structure in PM2.5. The results revealed thatProteobacteria was the predominant bacterial phylum comprising 94.3% of the total sequences, followed byFirmicutes,Actinobacteria,Bacteroidetes, andDeinococcus-Thermus. At the genus level, the top five abundant genera accounted for 81.8% of the total sequences. The proportions of major genera (Cupriavidus, Sphingobium, Brev...
Source: Aerobiologia - February 6, 2021 Category: Environmental Health Source Type: research
Bioaerosols in the landfill environment: an overview of microbial diversity and potential health hazards
AbstractLandfilling is one of the indispensable parts of solid waste management in various countries. Solid waste disposed of in landfill sites provides nutrients for the proliferation of pathogenic microbes which are aerosolized into the atmosphere due to the local meteorology and various waste disposal activities. Bioaerosols released from landfill sites can create health issues for employees and adjoining public. The present study offers an overview of the microbial diversity reported in the air samples collected from various landfill sites worldwide. This paper also discusses other aspects, including effect of meteo...
Source: Aerobiologia - February 4, 2021 Category: Environmental Health Source Type: research
Stochastic flowering phenology in Dactylis glomerata populations described by Markov chain modelling
AbstractUnderstanding the relationship between flowering patterns and pollen dispersal is important in climate change modelling, pollen forecasting, forestry and agriculture. Enhanced understanding of this connection can be gained through detailed spatial and temporal flowering observations on a population level, combined with modelling simulating the dynamics. Species with large distribution ranges, long flowering seasons, high pollen production and naturally large populations can be used to illustrate these dynamics. Revealing and simulating species-specific demographic and stochastic elements in the flowering process wi...
Source: Aerobiologia - February 3, 2021 Category: Environmental Health Source Type: research
Airborne pollen and spores ’ deposition in alveolar tissues as a tool in drowning forensic diagnosis
We report the results of a histological study of lung samples where an unusual quantity of airborne pollen and fungal spores was found in drowned rats. Pollen and spores were found in lungs of drowned rats but not in the post-mortem submerged ones. Another control group consisting of rats that underwent 60 min exposure to a highly pollen-loaded atmosphere also recorded negative for the presence of pollen or fungal spores. Pollen types coincided with plants growing at the surrounding gardens flowering during the days of the experiment, performed during spring, that were detected by the aerobiological trap located at the c...
Source: Aerobiologia - January 22, 2021 Category: Environmental Health Source Type: research
Germination dynamics of allergenic fungal spores in respiratory mucus
This study aimed to investigate the temporal germination dynamics of allergenicCladosporium, Aspergillus, andPenicillium spp. in respiratory mucus ex situ and the effect of the temperature (28 °C and 37 °C in the upper and lower respiratory tract, respectively) on fungal germination. Fungal spore germination was inhibited at 37 °C inC. oxysporum andC. cladosporioides, but notA. flavus,A. brunneoviolaceus,A. protuberus,P. citrinum, andP. oxalicum. Aspergillus flavus andA. brunneoviolaceus exhibited a high germination rate in the mucus at 28 °C and 37 °C. This indicated that their germination was not limited in t...
Source: Aerobiologia - January 20, 2021 Category: Environmental Health Source Type: research
Quantitative and qualitative assessment of microbial aerosols in different indoor environments of a dental school clinic
AbstractIn the indoor environment of dental clinics, dental staff and patients are exposed to various types of infectious agents transported by aerosols and particles, generated during dental procedures, promoting an increased risk of cross-infection. The aim of this study was to determine the levels and diversity of microbial aerosol in relation to particle load in five different departments of a dental school clinic. The air samples were collected by an active single-stage Andersen sampler during the treatment procedure. The mean concentrations of airborne bacteria were in the range of 52 –1030 and 8–844 CFU/m3 at t...
Source: Aerobiologia - January 13, 2021 Category: Environmental Health Source Type: research
Effect of selected sampling media, flow rate, and time on the sampling efficiency of a liquid impinger packed with glass beads for the collection of airborne viruses
AbstractThe liquid impingers can be used for sampling of viral aerosols, such as COVID-19 virus, influenza, and measles. However, the lowest cutoff diameter of commercially available liquid impingers was about 0.3 μm, and the physical collection efficiency for nano-bioaerosol is only about 10–20%. Here, we enhanced the impinger’s collection efficiency and recovery of viable viral aerosols by using packed glass beads and selected sampling media (1% peptone and lysogeny broth, LB). Single-stranded RNA (s sRNA) MS2 bacteriophage with uranine (as a physical tracer) was used as model viral aerosols. The effects of differ...
Source: Aerobiologia - January 13, 2021 Category: Environmental Health Source Type: research
Morning –evening variation and determinants of bacterial and fungal aerosol concentration in historical objects in Kraków
AbstractCultural heritage objects are very frequently visited by tourists, the presence of which is considered to affect the bacterial and fungal aerosol levels in these facilities. Therefore, this study had two main aims with the first to determine the morning/evening changes in the concentration of bacterial and fungal aerosol as well as particulate matter in the selected historical objects of Krak ów: churches, crypts, libraries, and museums. The second aim was to establish the most possible factors affecting the quality of air in the examined sites using data mining techniques: principal component analysis (PCA) and c...
Source: Aerobiologia - January 13, 2021 Category: Environmental Health Source Type: research
Properties relevant to atmospheric dispersal of the ice-nucleation active Pseudomonas syringae strain R10.79 isolated from rain water
AbstractTransport of microbes in the atmosphere allows them to spread and to colonize new habitats. To survive the harsh environmental conditions encountered in the atmosphere, these microorganisms have to possess properties that allow them to resist atmospheric stress. We combined physiological experiments and genome analysis ofPseudomonas syringae strain R10.79, previously isolated from rain, to decipher the strain ’s ability to withstand these challenges and identify the mechanisms behind the resistance. We used laboratory experiments to simulate different atmospheric stress factors and evaluated cell survival using f...
Source: Aerobiologia - January 13, 2021 Category: Environmental Health Source Type: research
Pollen Ole e 1 content variations in olive cultivars of different Portugal regions
AbstractOlive is recognized as a crop with great impact in agricultural, socioeconomic, environmental and public health sectors. The last is becoming more important during recent years as consequence of the increase of the pollen allergy in south Europe prompted by the widespreadOlea pollen allergic reactions. The aim of the study was to quantify, for the first time, the variations of the Ole e 1 allergen amount inOlea pollen grains from four cultivars in three regions of Portugal. How weather parameters can affect the allergen production was also assessed. The study was conducted in three olive producer areas of Portugal ...
Source: Aerobiologia - January 12, 2021 Category: Environmental Health Source Type: research
Efficacy of 75% alcohol in pretreatment of the Andersen sampler in trapping maximum airborne microbes
AbstractThe present study was conducted to evaluate the effects of the pretreatment methods and sampling time on the sampling of airborne bacteria in hospitals. Methods for the pretreatment of Andersen samplers, namely, non-sterilized, 75% ethanol and autoclaving sampled for 5 min, 10 min and 15 min in the general ward and class 1000 clean operating department, respectively, were studied. Statistical analysis was used to compare the differences in sampling results of airborne bacteria under different pretreatment methods, sampling time and environmental conditions. In the first test, the sampling results of the airborn...
Source: Aerobiologia - January 5, 2021 Category: Environmental Health Source Type: research
Correction to: Bioaerosol measurements over a fattening period in a pig barn focused on the presence of Staphylococcus spp.
In the original publication of the article, the below errors have occurred. (Source: Aerobiologia)
Source: Aerobiologia - January 3, 2021 Category: Environmental Health Source Type: research
Spatial distribution of atmospheric bioaerosols in Beijing, Hangzhou and Wuhan, China
In this study, air samples were collected in various planned areas of three cities in China (Beijing, Hangzhou and Wuhan) using a new liquid-based sampler. Animal, plant and bacterial components were quantified according to the expression levels of housekeeping genes via real-time PCR and analysis of cycle threshold (Ct) values. The level of animal-derived particles in the air of Hangzhou was higher than those in the other two cities. More bacterial particles than animal or plant particles were detected in the air in Beijing compared with other cities. We also found a negative correlation between the bacterial content of b...
Source: Aerobiologia - January 2, 2021 Category: Environmental Health Source Type: research
Assessment of indoor air quality in geriatric environments of southwestern Europe
The objective of this study was to evaluate physical –chemical and microbial indicators of indoor air quality in three nursing houses (NHs) located in rural areas of the northeast of Portugal. The parameters were measured during two campaigns (winter and summer), twice a day, and in four distinct spaces for spatial variability assessment: dining roo m, living room, double bedroom and outdoor of the NHs. Physical–chemical indicators were assessed by using a Graywolf IQ 610 probe. Airborne microbial levels were evaluated by direct impaction to the culture media, and subsequently, the microorganisms were identified molecu...
Source: Aerobiologia - January 1, 2021 Category: Environmental Health Source Type: research
