Erythropoietin-producing hepatocellular A7 restrains estrogen-negative feedback of luteinizing hormone via ephrin A5 in the hypothalamus of female rats.
In this study, we aimed to identify the mechanism and hypothalamus-pituitary-ovary (HPO) axis level underlying the promotion of LH secretion by EPHA7. Using an ovariectomized (OVX) rat model, in conjunction with low-dose estradiol (E2) treatment, we investigated the association between EPHA7-ephrin (EFN) A5 signaling and E2-negative feedback. Various rat models (OVX, E2-treated OVX, and abarelix-treated) were injected with the recombinant EPHA7-Fc protein through the caudal vein to investigate the molecular mechanism underlying the promotion of LH secretion by EPHA7. Efna5 was observed strongly expressed in the arcuate nuc...
Source: American Journal of Physiology. Endocrinology and Metabolism - May 11, 2020 Category: Physiology Authors: Li S, Zhai J, Xu B, Liu J, Chu W, Wang D, Geng X, Chen ZJ, Du Y Tags: Am J Physiol Endocrinol Metab Source Type: research
In-silico driven engineering of enantioselectivity of a penicillin G acylase towards active pharmaceutical ingredients
In this study, we have evaluated effects of in-silico replacements of acyl-binding subsite residue Phe24β of the enzyme from Achromobacter sp. CCM 4824 to seven markedly smaller amino acids on its enantioselectivity towards industrially relevant compounds. Models of the two most promising mutants bearing substitutions Pheβ24Ala and Pheβ24Cys were investigated using molecular docking calculations. The Cys substitution revealed much better enantioselectivity traits with a set of seven substrates. To verify the relevance of in-silico predictions, we constructed a PGA A + Phe24βCys mutant and determined its enantioselecti...
Source: Journal of Molecular Catalysis B: Enzymatic - November 14, 2016 Category: Biochemistry Source Type: research
Utility of LHRH antagonists for advanced prostate cancer.
CONCLUSIONS: The rapid T-suppression achieved with degarelix may provide a clinical benefit for various groups of men with advanced or locally advanced disease. PMID: 24775720 [PubMed - in process] (Source: Canadian Journal of Urology)
Source: Canadian Journal of Urology - December 1, 2014 Category: Urology & Nephrology Tags: Can J Urol Source Type: research