A neglected challenge in analysis of active pharmaceutical substances with aldehyde functional group in aqueous matrices

Aldehydes are compounds that are widely used and popular in organic synthesis due to their high reactivity. However, due to the ability of aldehydes to participate in nucleophilic addition reactions, especially in biological aqueous media, drugs with aldehydic functional groups should be always used with caution and carefully quantified in biological fluids.Our experience in working on biologically active aldehydes indicates the transformation of these group of compounds in aqueous or alcoholic solution and thus the failure of analytical methods for their accurate monitoring in such media specially when mass spectrometry is the method of detection.Both mass spectrometric and 1H-NMR spectroscopic evidences indicate the reaction of spiramycin with water molecules in aqueous solution which results in conversion of spiramycin to a new molecule with 18 mass unit difference and thus the residue amount which is measured and reported based on a mass spectrometric method, does not show the correct amount of spiramycin in these products.
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research