Profiling Protein-DNA Interactions Cell-Type-Specifically with Targeted DamID

Methods Mol Biol. 2022;2458:195-213. doi: 10.1007/978-1-0716-2140-0_11.ABSTRACTTargeted DamID (TaDa) is a means of profiling the binding of any DNA-associated protein cell-type specifically, including transcription factors, RNA polymerase, and chromatin-modifying proteins. The technique is highly sensitive, highly reproducible, requires no mechanical disruption, cell isolation or antibody purification, and can be performed by anyone with basic molecular biology knowledge. Here, we describe the TaDa method and downstream bioinformatics data processing.PMID:35103969 | DOI:10.1007/978-1-0716-2140-0_11

Source: Mol Biol Cell - February 1, 2022 Category: Molecular Biology Authors: Owen J Marshall Caroline Delandre Source Type: research