Transcriptomic analysis of Streptococcus agalactiae periprosthetic joint infection

Transcriptomic analysis ofStreptococcus agalactiae periprosthetic joint infection (PJI) was performed using fluid derived from sonication of explanted arthroplasties of subjects withS. agalactiae PJI. Results were compared to those ofS. agalactiae strain NEM316 grownin vitro. Results of functional enrichment analysis are shown; one interesting finding was that genes involved in inorganic ion transport and metabolism were enriched in PJI. AbstractAlthoughStreptococcus agalactiae periprosthetic joint infection (PJI) is not as prevalent as staphylococcal PJI, invasiveS. agalactiae infection is not uncommon. Here, RNA-seq was used to perform transcriptomic analysis ofS. agalactiae PJI using fluid derived from sonication of explanted arthroplasties of subjects withS. agalactiae PJI, with results compared to those ofS. agalactiae strain NEM316 grown in vitro. A total of 227 genes with outlier expression were found (164 upregulated and 63 downregulated) between PJI sonicate fluid and in vitro conditions. Functional enrichment analysis showed genes involved in mobilome and inorganic ion transport and metabolism to be most enriched. Genes involved in nickel, copper, and zinc transport, were upregulated. Among known virulence factors,cyl operon genes, encoding β-hemolysin/cytolysin, were consistently highly expressed in PJIversus in vitro. The data presented provide insight intoS. agalactiae PJI pathogenesis and may be a resource for identification of novel PJI therapeutics or vaccine...

Source: MicrobiologyOpen - December 17, 2021 Category: Microbiology Authors: Hye ‐Kyung Cho, Thao Masters, Kerryl E. Greenwood‐Quaintance, Stephen Johnson, Patricio R. Jeraldo, Nicholas Chia, Meng Pu, Matthew P. Abdel, Robin Patel Tags: ORIGINAL ARTICLE Source Type: research