Cryoprotectant-free preservation of bacteria using semi-spherical drops

Cryobiology. 2021 Nov 25:S0011-2240(21)00354-0. doi: 10.1016/j.cryobiol.2021.11.179. Online ahead of print.ABSTRACTCryopreservation is a widely used long-term preservation method to ensure the quality and vitality of microbes in laboratories and biological resource facilities. However, freeze-thaw damage and osmotic pressure changes during cryopreservation adversely impacts microbial survival. Significant expenditure of resources and expertise are required to select the right cryoprotectant and optimize its concentration for maximum survival of diverse microorganisms. This work describes a cryopreservation method that obviates the need for cryoprotectants by exploiting the unique thermal characteristics of semi-spherical drops. Here, a plurality of these drops, each 10 μl in volume, created on a highly non-wetting flat-sheet substrate with holes and frozen at -70 °C. Deriving an f (x) metric as a measure of relative viability, storage in drops in the absence of cryoprotectants was found to improve the survivability of Staphylococcus epidermidis by 1.91 times compared with the same sample stored in larger 50-μl volumes in standard 1.5-ml tubes. This also compares well with a value of 2.33 obtained with standard preservation with cryoprotectant. The drop method allows high throughput aliquoting of the bacterial culture into multiple discrete drops using multichannel pipettes or automated liquid handlers and the edges of the holes provides a pinning action that holds t...
Source: Cryobiology - Category: Biology Authors: Source Type: research

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AbstractBackgroundDesmopressin acetate was recommended for nocturia in benign prostatic hyperplasia (BPH) patients recently, but its effect and safety is still controversial. We aimed to establish a systematic review and meta-analysis to confirm its effect on symptom relief and adverse effects.MethodsA systematic search was performed in PubMed, Cochrane Library, EMBASE, Medline, Web of Science and Science Direct databases from January 2000 to October 2021 for controlled trials of BPH patients comparing oral desmopressin with control groups. The mean difference (MD) and odds ratio (OR) were meta-analyzed.ResultsFour article...
Source: LUTS: Lower Urinary Tract Symptoms - Category: Urology & Nephrology Authors: Tags: ORIGINAL ARTICLE Source Type: research
Chemistry of MaterialsDOI: 10.1021/acs.chemmater.1c03253
Source: Chemistry of Materials - Category: Materials Science Authors: Source Type: research
To examine whether a history of nocturnal enuresis affects nighttime urinary frequency and to evaluate nocturia etiologies in adults.
Source: Urology - Category: Urology & Nephrology Authors: Tags: Original Article Source Type: research
Condition:   Enuresis Intervention:   Device: uroflowmetry Sponsor:   Hisar Intercontinental Hospital Completed
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Curr Sports Med Rep. 2022 Jan 1;21(1):15-17. doi: 10.1249/JSR.0000000000000926.ABSTRACTA 49-year-old woman with a history of daily inhaled corticosteroid use for asthma presented to a concussion clinic 7 wk after sport-related head injury with headache, visual blurring, dizziness, nausea, fatigue, polydipsia, and polyuria. Examination revealed difficulty with vestibulo-occulomotor testing due to nausea and visual straining. Cranial CT/MR imaging was unremarkable. Laboratory testing revealed critically low serum cortisol, hypernatremia, and urine studies suggesting diabetes insipidus. The patient was referred to the emergen...
Source: Current Sports Medicine Reports - Category: Sports Medicine Authors: Source Type: research
In this study we investigated and characterized the molecular and pathogenic mechanisms through which a novel cysteine variant p.(Cys1084Tyr) causes an unusual, mixed phenotype form of von Willebrand disease (VWD). Phenotypic data including bleeding scores, laboratory values, VWF multimer distribution and desmopressin response kinetics were investigated in 5 members (2 parents and 3 daughters) of a consanguineous family. VWF synthesis and secretion were also assessed in a heterologous expression system and in a transient transgenic mouse model. Heterozygosity for p.(Cys1084Tyr) was associated with variable expressivity of ...
Source: Adv Data - Category: Epidemiology Authors: Source Type: research
Curr Sports Med Rep. 2022 Jan 1;21(1):15-17. doi: 10.1249/JSR.0000000000000926.ABSTRACTA 49-year-old woman with a history of daily inhaled corticosteroid use for asthma presented to a concussion clinic 7 wk after sport-related head injury with headache, visual blurring, dizziness, nausea, fatigue, polydipsia, and polyuria. Examination revealed difficulty with vestibulo-occulomotor testing due to nausea and visual straining. Cranial CT/MR imaging was unremarkable. Laboratory testing revealed critically low serum cortisol, hypernatremia, and urine studies suggesting diabetes insipidus. The patient was referred to the emergen...
Source: Current Sports Medicine Reports - Category: Sports Medicine Authors: Source Type: research
In this study, highly reducible CeO2-x nanoparticles are synthesized aiming to provide Cu/CeO2-x nanoparticles, which are classically active catalysts for the CO oxidation reaction. It is observed that the Cu nanoparticles shape changes during reduction treatment (prior to the CO oxidation reaction) from a nearly spherical 3D to a planar 2D shape, then enhances the Cu –CeO2-x interaction. The spread of the Cu nanoparticles over the CeO2-x surface during the reduction treatment occurs due to the minimization of the total system energy. The shape change is accompanied by migration of O atoms from CeO2 surface to the bo...
Source: Small - Category: Nanotechnology Authors: Tags: Research Article Source Type: research
J. Mater. Chem. A, 2022, Accepted Manuscript DOI: 10.1039/D1TA07695G, PaperLi Liu, Laura Charlton, Yanqing Song, Tao Li, Xue-Mei Li, Huabing Yin, Tao He Membrane distillation is a thermally driven separation process using hydrophobic, porous membranes. Among various problems faced by membrane distillation, scaling remains an unresolved challenge in treating streams of high salinity.... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - J. Mater. Chem. latest articles - Category: Chemistry Authors: Source Type: research
Faraday Discuss., 2022, Advance Article DOI: 10.1039/D1FD00048A, PaperSeung-Ryong Kwon, Seol Baek, Paul  W. Bohn Potential-induced wetting and dewetting behaviors in pH-responsive block copolymer membranes are investigated to establish a promising mass transport control system for ultrasensitive sensing applications. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Faraday Discuss. latest articles - Category: Chemistry Authors: Source Type: research
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