Distinct microbiota assembly mechanisms revealed in different reconstruction stages during gut regeneration in the sea cucumber Apostichopus japonicus

Ejection of internal organs was induced in the sea cucumberApostichopus  japonicus, and gut immune responses and bacterial diversity during gut regeneration were investigated. We revealed that the reconstruction of the bacterial community involved two stages. The diversity, taxonomic composition, and bacterial interaction of the gut bacterial community and the interaction between the bacterial community and the host were significantly different in the two stages. AbstractApostichopus  japonicus is a useful model for studying organ regeneration, and the gut microbiota is important for host organ regeneration. However, the reconstruction process and the mechanisms of gut microbiota assembly during gut regeneration in sea cucumbers have not been well studied. In the present study, gut regeneration was induced (via evisceration) inA.  japonicus, and gut immune responses and bacterial diversity were investigated to reveal gut microbiota assembly and its possible mechanisms during gut regeneration. The results revealed that bacterial community reconstruction involved two stages with distinct assembly mechanisms, where the reconstructed community was initiated from the bacterial consortium in the residual digestive tract and tended to form a novel microbiota in the later stage of reconstruction. Together, the results of immunoenzyme assays, community phylogenetic analysis, and source tracking suggested that the host deterministic process was stronger in the initial stage than in ...

Source: MicrobiologyOpen - November 23, 2021 Category: Microbiology Authors: Zichao Yu, Zhuang Xue, Chao Liu, Anguo Zhang, Qiang Fu, Kun Yang, Fang Zhang, Liyuan Ran Tags: ORIGINAL ARTICLE Source Type: research