Platinated oligomers of bovine pancreatic ribonuclease: Structure and stability.

Platinated oligomers of bovine pancreatic ribonuclease: Structure and stability.
J Inorg Biochem. 2015 Feb 26;146:37-43
Authors: Picone D, Donnarumma F, Ferraro G, Russo Krauss I, Fagagnini A, Gotte G, Merlino A
Abstract
The reaction between cis-diamminedichloroplatinum(II) (CDDP), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease (RNase A), induces extensive protein aggregation, leading to the formation of one dimer, one trimer and higher oligomers whose yields depend on cisplatin/protein ratio. Structural and functional properties of the purified platinated species, together with their spontaneous dissociation and thermally induced denaturation, have been characterized. Platinated species preserve a significant, although reduced, ribonuclease activity. The high resistance of the dimers against dissociation and the different thermal unfolding profiles suggest a quaternary structure different from those of the well-known swapped dimers of RNase A.
PMID: 25756333 [PubMed - as supplied by publisher]

Source: Journal of Inorganic Biochemistry - February 26, 2015 Category: Biochemistry Authors: Picone D, Donnarumma F, Ferraro G, Russo Krauss I, Fagagnini A, Gotte G, Merlino A Tags: J Inorg Biochem Source Type: research