EP News: Basic and Translational

In the current study, Harley et al (Proc Natl Acad Sci U S A 2021 ;118(44):e2108796118; PMID: 34716268) investigated the roles of the cytosolic assembly formed by the Per-Arnt-Sim (PAS) and cyclic nucleotide binding homology (CNBh) domains of the hERG K+ channel in channel gating, using single-chain variable fragment (scFv) antibodies. The authors mapped the scFv2.12 epitope to a site overlapping with the PAS/CNBh domain interface. ScFv binding in vitro and in the cell is incompatible with the PAS interaction with CNBh.

Source: Heart Rhythm - November 10, 2021 Category: Cardiology Authors: Nipavan Chiamvimonvat Source Type: research