Efficient enzymatic synthesis of L ‐ascorbyl palmitate using Candida antarctica lipase B‐embedded metal‐organic framework

AbstractTheCandida antarctica lipase B (CALB) was embedded in the metal-organic framework, zeolitic imidazolate framework-8 (ZIF-8), and applied in the enzymatic synthesis ofL-ascorbic acid palmitate (ASP) for the first time. The obtained CALB@ZIF-8 achieved the enzyme loading of 80  mg g−1 with 11.3  U g−1 (dry weight) unit activity, 59.8% activity recovery, and 92.7% immobilization yield. Under the optimal condition, ASP was synthesized with over 75.9% conversion ofL-ascorbic acid in a 10-batch reaction. Continuous synthesis of ASP was subsequently performed in a packed bed bioreactor with an outstanding average space –time yield of 58.1 g L−1 h−1, which was higher than ever reported continuous ASP biosynthesis reactions.

Source: Biotechnology Progress - October 18, 2021 Category: Biotechnology Authors: Xiao ‐Jian Zhang, Feng‐Yu Qi, Jia‐Mei Qi, Fei Yang, Jiang‐Wei Shen, Xue Cai, Zhi‐Qiang Liu, Yu‐Guo Zheng Tags: RESEARCH ARTICLE Source Type: research