Synergistic effect of thymoquinone and nystatin in the treatment of oral candidiasis; an in vitro study

AbstractThe effectiveness of antifungal agents may be insufficient against resistant strains in some cases of oral candidiasis. The aim of this study was to evaluate the antifungal effect of thymoquinone againstCandidaalbicans,Candidatropicalis,Candidaglabrata andCandidakrusei strains and the synergistic antifungal activity of these strains in combination with nystatin. To evaluate in vitro antifungal activity and interactions between thymoquinone and nystatin, substances were tested againstCandidaalbicans ATCC 10,231,C.tropicalis ATCC 750,C.krusei ATCC 6258 andC.glabrata ATCC 2001 standard strains both individually and combinationally via microdilution method. MIC and ΣFIC index value were analysed. The Kruskal Wallis test and Bonferroni test were used for statistical evaluations. Statistical significance was set atp <  0.05. A statistically significant difference was observed between the mean ranks of all Candida species and doses of thymoquinone, nystatin, and the combination thymoquinone-nystatin (p <  0.05). MIC values for thymoquinone were determined as 15 μg/mL forC.albicans,C.tropicalis andC.krusei while it was 30  μg/mL forC.glabrata. Moreover, MIC for nystatin was found as 1.875  μg/mL forC.albicans,C.tropicalis andC.krusei, whereas it was 7.5  μg/mL in C. glabrata. Interaction assays and ΣFIC index value revealed that, TQ and nystatin have a synergistic effect against to all strains. Thymoquinone was found to have antifungal activity on Cand...
Source: Odontology - Category: Dentistry Source Type: research