Indigofera tinctoria leaf powder as a promising additive to improve indigo fermentation prepared with sukumo (composted Polygonum tinctorium leaves)

AbstractBeing insoluble in the oxidize form, indigo dye must be solubilized by reduction for it to penetrate textile. One of the procedures is the reduction by natural bacterial fermentation.Sukumo, composted leaves ofPolygonum tinctorium, is a natural source of indigo in Japan. Althoughsukumo has an intrinsic bacterial seed, the onset of indigo reduction with this material may vary greatly. Certain additives improve indigo fermentation. Here, we studied the effects ofIndigofera tinctoria leaf powder (LP) on the initiation of indigo reduction, bacterial community, redox potential (ORP), and dyeing intensity in the initial stages and in aged fermentation fluids prepared withsukumo.I.tinctoria LP markedly decreased ORP at day 1 and stabilised it during early fermentation. These effects could be explained by the phytochemicals present inI.tinctoria LP that act as oxygen scavengers and electron mediators. Using next generation sequencing results, we observed differences in the bacterial community insukumo fermentation treated withI.tinctoria LP, which was not influenced by the bacterial community inI.tinctoria LP per se. The concomitant decrease inBacillaceae and increase inProteinivoraceae at the onset of fermentation, increase in the ratio of facultative to obligate anaerobes (F/O ratio), or the total abundance of facultative anaerobes (F) or obligate anaerobes (O) (designated F  + O) are vital for the initiation and maintenance of indigo reduction. Hence,I.tinctoria LP imp...
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research