Microbial factories: Monitoring vitamin B2 production by Escherichia coli in microfluidic cultivation chambers

This article is licensed under aCreative Commons Attribution-NonCommercial 3.0 Unported Licence.Petra Juskova, Steven Schmitt, Lucas Armbrecht, Petra S Dittrich Microbial cells represent a standard production host for various important biotechnological products. Production yields can be increased by optimising strains, growth conditions and understanding deviations in production rates over time... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Lab Chip latest articles - Category: Chemistry Authors: Source Type: research

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ConclusionThe recommended IV dose of 10  mg/kg q8h, not exceeding 400 mg q8h, would achieve AUC0-24  h to successfully treat bacteria with MICs  ≤ 0.25 (e.g. Salmonella, Escherichia coli, Proteus, Haemophilus, Enterobacter, and Klebsiella). A dose increase to 600 mg q8h in children >  40 kg and to 15 mg/kg q8h (max 400 mg q8h, max 600 mg q8h if augmented renal clearance, i.e., eGFR >  200 mL/min/1.73 m2) in children  
Source: European Journal of Clinical Pharmacology - Category: Drugs & Pharmacology Source Type: research
Green Chem., 2021, Advance Article DOI: 10.1039/D1GC02867G, PaperJian Wang, Ruihua Zhang, Jianli Zhang, Xinyu Gong, Tian Jiang, Xinxiao Sun, Xiaolin Shen, Jia Wang, Qipeng Yuan, Yajun Yan To achieve low-carbon bioeconomy, we established a carbon-efficient bioproduction approach by fine-tuning a non-glycolysis pathway, which reduces CO2 emission and balances the acetyl-CoA and redox supply from sugar breakdown to pathway demand. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Green Chem. latest articles - Category: Chemistry Authors: Source Type: research
In this study, it was aimed to evaluate the EUCAST RAST method in blood culture bottles that resulted with positive signal in BacT/ALERT (bioMérieux, France) blood culture system and that showed gram-negative bacteria with single morphology with Gram stain. The study was conducted prospectively between April 2019 and November 2019. Ninety blood culture bottles that we detected single gram negative bacteria morphology by Gram stain were tested according to the EUCAST RAST method, The isolates obtained from the blood culture bottles were studied with the EUCAST disk diffusion method and the Vitek 2 Compact (bioMerieux...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Authors: Source Type: research
In this study, it was aimed to evaluate the utility of disk diffusion, agar dilution kit and automated MicroScan methods (Beckman Coulter, Brea, CA, ABD) for fosfomycin susceptibility of ESBL-producing enteric bacteria. The study included 120 ESBL positive isolates of enteric bacteria (89 Escherichia coli, 31 Klebsiella pneumoniae) cultivated from the samples sent to the Microbiology Laboratory of the Mersin University Medical Faculty of Medicine Hospital between May 2019 and November 2019. Fosfomycin susceptibilities of the isolates included in the study were tested by disk diffusion, automated MicroScan and the commercia...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Authors: Source Type: research
In this study, it was observed that rotavirus, which is the cause of childhood diarrhea, can also be encountered in advanced ages and even new genotypes that infect humans worldwide may also be the causative agents. Therefore, we concluded that it is important to investigate new genotypes such as G10 and G12 in molecular epidemiological studies.PMID:34666655 | DOI:10.5578/mb.20219707
Source: Mikrobiyoloji Bulteni - Category: Microbiology Authors: Source Type: research
In this study, we aimed to evaluate the susceptibility of Salmonella and Shigella species to azithromycin, to determine and compare the minimum inhibitory concentration (MIC) values and disk diffusion zone diameters. In addition, susceptibility to meropenem and first-line antibiotic options in isolates was also investigated. A total of 170 Salmonella, 76 Shigella clinical isolates collected between 2014 and 2018 in our hospital were tested for their susceptibility to azithromycin, meropenem, ampicillin, pefloxacin, trimetoprim-sulfamethoxazole, ceftazidime, and cefotaxime. Isolates were identified by matrix-assisted laser ...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Authors: Source Type: research
Conclusions: This study revealed a high prevalence of infection with at least one or six non‑pathogenic and pathogenic intestinal protozoa in UC patients in the Isfahan region. Intestinal protozoa are a challenging public health problem wherever health care is limited in the area. The emergence of UC in the world result s in the need to study etiologic factors. In order to obtain further information about the etiology of disease, we investigated the prevalence of intestinal protozoan infection in patients with UC in Isfahan, Iran.
Source: International Journal of Preventive Medicine - Category: International Medicine & Public Health Source Type: research
ConclusionsSTEC are a heavy hazard for the consumers and the number of cases is slightly increasing since 2015. Our results highlight a low number of presumptive positive samples and even lower number of STEC detection obtained with the ISO classical method. Furthermore, detection of the target genes in broth was rarely confirmed after plating, highlighting a weak point of the analytical method that needs to be investigated. Efforts are necessary to improve the method and the STEC confirmation from colonies. Immunomagnetic beads targeting the top five serogroups and digital PCR could help in increasing the recovery rate of...
Source: The European Journal of Public Health - Category: General Medicine Source Type: research
AbstractIntroductionThe state of Amazonas is the largest state in Brazil and it has 62 counties, including Manaus, its capital, all of which have plenty of water resources. The control and surveillance of water quality, as well as the sewage treatment must be carried out satisfactorily in order to avoid a favorable environment for the development of pathogens that cause water related diseases.ObjectivesAssess the quality of the water supplied to the population of the 62 counties in the period of 2015-2019.MethodsQuantitative and qualitative descriptive study, based on secondary data taken from Water Quality Surveillance In...
Source: The European Journal of Public Health - Category: General Medicine Source Type: research
This study reports the identification of a new post-translational modification, termed ADP riboxanation, which is mediated by the Shigella effector OspC3 and inactivates the cytosolic LPS sensing pathway of caspase-4 and caspase-11.
Source: Nature AOP - Category: Research Authors: Source Type: research
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