P –040 Correlation between DNA fragmentation index and big halo pattern with sperm decondensation index and semen sample parameters

AbstractStudy questionDoes sperm DNA integrity evaluated by DNA fragmentation index (DFI) and big halo pattern correlate with sperm decondensation index (SDI) and semen sample parameters?Summary answerDFI correlates with SDI and semen sample parameters in a stronger way than the big halo patternWhat is known alreadyThe sperm chromatin dispersion test evaluates DNA integrity by measuring the susceptibility of sperm DNA containing breaks to denature when treated by an acid solution. Spermatozoa with intact DNA produce big or medium size halos of dispersed DNA loops, whereas small halos or no halos indicate fragmented DNA. The DFI calculates the proportion of spermatozoa with fragmented DNA. Data have been published documenting the negative effect of sperm DFI on embryo viability, suggesting that its evaluation could contribute to the prediction of the male reproductive potentialStudy design, size, durationA prospective study between 2011 to 2019 included 300 patients attending our clinic for fertility treatment. All sperm samples were analyzed according to WHO criteria, and the results from the DNA integrity analysis were related to the semen sample indicesParticipants/materials, setting, methodsOf the 300 males included in the study, 118 were normozoospermic, 16 were oligozoospermic (O), 63 were asthenozoospermic (A), 9 were teratozoospermic, 7 were AT, 51 were OA, 5 were OT, and 31 OAT. The DNA integrity was assessed by the Halosperm test, and DNA decondensation by the anilin...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research