O-087 Embryos with higher mitochondrial DNA ratios show better clinical outcomes in single euploid embryo transfer

This study is a retrospective cohort study from 2016 to 2019, including 1465 single embryo transfer cycles.Participants/materials, setting, methodsThe involved embryos were biopsied on Day 5 or 6 and the mitochondrial DNA ratio of 1465 embryos was examined undergoing PGS/NGS. The mitochondrial DNA ratios were normalized for technical batch-to-batch variation. The mitochondrial DNA ratio between the implantation group and non-implantation group was statistically analyzed. Data were analyzed by the student ’s t-test for continuous variables and Chi-square test for categorical variables.Main results and the role of chanceThe mitochondrial DNA ratio of embryos was no significant difference between different age spans ( p  = 0.772) and ploidy (p = 0.224). D5 biopsied embryos, however, contained a significantly higher mitochondrial DNA ratio than D6 biopsied embryos (p <  0.0001). All of the single embryo transferred embryos were classified into two groups; implanted and non-implanted embryos. Results from 1465 transferred embryos show that the mitochondrial DNA ratio of implanted embryos was statistically significantly higher than non-implanted embryos (p = 0 .0053). Besides, the cut-off values were established, dividing the transferred embryos into high and low mitochondrial DNA ratio groups. The pregnancy rate and implantation rate of the high mitochondrial DNA ratio group was higher than the low mitochondrial DNA ratio group: [Pregnancy rate] 74% vs. 63.5% ...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research