Colorimetric and fluorometric dual-readout protein kinase assay by tuning the active surface of nanoceria

Chem Commun (Camb). 2021 Jul 27. doi: 10.1039/d1cc03357c. Online ahead of print.ABSTRACTHerein, we demonstrate that the active surface of nanoceria can be fine-tuned by phosphorylated peptides. Accordingly, a colorimetric and fluorometric dual-readout strategy is rationally developed for assaying protein kinase activity. This feature not only enables the versatile monitoring of peptide phosphorylation but also broadens the application scope of nanoceria.PMID:34313270 | DOI:10.1039/d1cc03357c
Source: Chemical Communications - Category: Chemistry Authors: Source Type: research