GSE180131 Sequencing of siRNA from soybean containing silencing vectors and empty vectors

Contributors : Megan McCaghey ; Dandan Shao ; Kurcezewski Kurcezewski ; Ally Lindstrom ; Ashish Ranjan ; Steven Whitham ; Shawn Conley ; Brett Williams ; Damon Smith ; Mehdi KabbageSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Glycine maxA cDNA library was constructed by Novogene (CA, USA) using a Small RNA Sample Pre Kit, and Illumina sequencing was conducted according to company workflow, using 20 million reads. Raw data were filtered for quality as determined by reads with a quality score> 5, reads containing N< 10%, no 5' primer contaminants, and reads with a 3' primer and insert tag. The 3' primer sequence was trimmed and reads with a poly A/T/G/C were removed
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Glycine max Source Type: research