Phosphorus and sulfur SAD phasing of the nucleic acid-bound DNA-binding domain of interferon regulatory factor 4

This article reports the use of native intrinsic phosphorus and sulfur single-wavelength anomalous dispersion methods to solve the complex of the DNA-binding domain (DBD) of interferon regulatory factor 4 (IRF4) bound to its interferon-stimulated response element (ISRE). The structure unexpectedly shows three molecules of the IRF4 DBD bound to one ISRE. The sole reliance on native intrinsic anomalous scattering elements that belong to DNA – protein complexes renders the method of general applicability to a large number of such protein complexes that cannot be solved by molecular replacement or by other phasing methods.

Source: Acta Crystallographica Section F - June 29, 2021 Category: Biochemistry Authors: Agnarelli, A. El Omari, K. Duman, R. Wagner, A. Mancini, E.J. Tags: experimental phasing native SAD phosphorus DNA DNA-binding proteins IRF4 interferon regulatory factor 4 research communications Source Type: research