Platelet-rich plasma alleviates myocardial ischemia-reperfusion injury in rats
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):775-782. doi: 10.12122/j.issn.1673-4254.2021.05.20.ABSTRACTOBJECTIVE: To investigate the protective effect of platelet-rich plasma (PRP) against acute myocardial ischemiareperfusion (IR) injury and the possible mechanism.OBJECTIVE: Aortic blood samples were collected from 10 SD rats to prepare PRP, in which the concentrations of platelet-derived growth factor-BB (PDGF-BB) and transforming growth factor-β1 (TGF-β1) were measured. Cell models of IR injury were established in primary cultures of neonatal SD rat cardiomyocytes by exposing the cells to 3 h of hypoxia. The cells were then reoxygenated and co-cultured with 1%, 5%, 10%, and 20% volume of PRP for 12 h, and the changes in cell viability was assessed. Immunofluorescence staining of the cardiomyocytes was performed, and the cellular expression of AMPK and its phosphorylation level were detected. The effects of PRP on the proliferation and migration of rat aortic endothelial cells (RAOECs) were examined. In a SD rat model of myocardial IR injury, 100 μL of PRP (n= 20) or normal saline (n=20) was injected at 4 sites around the ligation site immediately after cardiac reperfusion. One day after the injection, 6 rats were selected from each group for TTC staining of the myocardial tissues and measurement of troponin Ⅰ content. One week later, the cardiac function of the remaining rats was assessed by echocardiography, and HE staining of the myocardial tissues was...
This article aims to introduce forensic practitioners to basic concepts in genetics and epigenetics so that they are able to engage with the relevant literature and understand the far-reaching implications for forensic practice. It is becoming increasingly useful for forensic practitioners to appreciate how life experiences are encoded into biology through epigenetics. This paper highlights the potential of genetic and epigenetic research to provide major contributions to real-world practice in the coming years. It provides a modern biopsychosocial perspective on harmful behaviour and helps deepen the understanding of o...
In the article, “Nutritional Management for Dogs and Cats with Chronic Kidney Disease,” by Valerie J. Parker, published in the May 2021 issue (Volume 51, number 3, pages 685-710), in Table 3 on page 12, for the “2020 AAFCO Feline adult maintenance maximum requirement” under the “Vitamin D (IU)”, the co rrect number should be 752 instead of 75.2.
Diagnostic Imaging: Point-of-Care Ultrasound
Christopher A. Adin and Kelly D. Farnsworth
CHRISTOPHER A. ADIN, DVM
VETERINARY CLINICS OF NORTH AMERICA: SMALL ANIMAL PRACTICE
This article discusses ethical considerations and principles and propose use of the 4Es model and core communication skills to address ethical dilemmas in veterinary practice. It reviews literature defining ethical issues in practice and provides case examples to show the application of our proposed methods. The goal is to provide veterinary professionals with an approach they can use to frame and address their own ethical decisions.
One of the most impactful ways to create a dynamic team is to foster diversity and inclusivity within the workplace. Workplaces have become more heterogenous as advances in human, women, and civil rights group have spurred greater labor force participation by members of historically underrepresented groups. Studies have shown that leveraging diversity has important implications for the promotion of positive organization change through facilitation of individual and organization performance. Diverse clientele may be more comfortable and feel more welcome working with people in a diverse workplace.
The Trevose company was at one time talking with nine interested parties about a deal.