Optimization of (-)-cubebin biotransformation to (-)-hinokinin by the marine fungus Absidia coerulea 3A9

This study evaluates the ability of the Absidia coerulea 3A9 marine derived strain isolated from the ascidian Distaplia stilyfera to perform biotransformations by conducting assays with (-)-cubebin, as substrate. The experiment was optimized using the experimental design proposed by Plackett-Burman for seven factors and eight experiments, to establish the biotransformation conditions that would allow maximum production of biotransformed dibenzylbutyrolactone (-)-hinokinin. An analytical method based on Reverse-Phase-High Performance Liquid Chromatography (RP-HPLC) was developed to quantify the fungal biotransformation product. The factor that influenced the (-)-hinokinin peak area the most positively was the percentage of seawater (%seawater) given that its %relative standard deviation (%RSD) showed a 32.92% deviation from the real value.PMID:34110481 | DOI:10.1007/s00203-021-02417-0

Source: Archives of Microbiology - June 10, 2021 Category: Microbiology Authors: Jonathan Messias de Souza Mario Ferreira Concei ção Santos Rita Cassia Nascimento Pedroso Leticia Pereira Pimenta K átia Aparecida Siqueira Marcos Antonio Soares Gustavo Muniz Dias Rosemeire Cristina Linhari Rodrigues Pietro Henrique Pereira Ramos Marc Source Type: research