In vitro toxicological evaluation of arsenic on superoxide dismutase mediated oxidative stress in renal cells of rat and its amelioration with supplementation of Pleurotus florida lectin (PFL)

AbstractConcomitant oral supplementation of Pleurotus florida lectin (PFL) (@50 μg/ml) on arsenic (@ 5 μM) exposed renal cells was investigated to evaluate the protective value of PFL against arsenic toxicity in rats. Renal cells, exposed to arsenic(III), showed a significant inhibition of cell proliferation index (CPI) and superoxide dismutase (SOD) acid and catalase (CAT) a ctivity with an increased level of lipid peroxidation (LPO). A marginally increased level of nitric oxide (NO) was found firstly and thereafter decreased due to long-term arsenic exposure in the cells. Concurrent application of PFL in arsenic exposed renal cells had remarkable protective effect on i nhibited CPI, SOD, and CAT activity and restored LPO and NO level in renal cells. Arsenic caused down-regulation of mRNA expression of SOD2 gene that was reversed by administration of PFL. The results concluded that supplementation of PFL protected renal cells against arsenic-induced oxidative stress.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research