Miltefosine is active against Sporothrix brasiliensis isolates with in vitro low susceptibility to amphotericin B or itraconazole.

Miltefosine is active against Sporothrix brasiliensis isolates with in vitro low susceptibility to amphotericin B or itraconazole.
J Med Microbiol. 2015 Feb 13;
Authors: Borba-Santos LP, Gagini T, Ishida K, de Souza W, Rozental S
Abstract
Sporotrichosis is a common mycosis caused by dimorphic fungi from the Sporothrix schenckii complex. In recent years, sporotrichosis incidence rates have increased in the Brazilian state of Rio de Janeiro, where Sporothrix brasiliensis is the species more frequently isolated from patients. The standard antifungals itraconazole and amphotericin B are recommended as first-line therapy for cutaneous/lymphocutaneous and disseminated sporotrichosis, respectively, although decreased sensitivity to these drugs in vitro was reported for clinical isolates of S. brasiliensis. Here, we evaluated the activity of the phospholipid analogue miltefosine - already in clinical use against leishmaniasis - towards the pathogenic yeast form of S. brasiliensis isolates with low sensitivity to itraconazole or amphotericin B in vitro. Miltefosine had fungicidal activity, with minimum inhibitory concentration (MIC) values between 1-2 µg.mL-1. Miltefosine exposure led to loss of plasma membrane integrity, and transmission electron microscopy (TEM) analysis revealed decrease in cytoplasmic electron density, alterations in the thickness of cell wall layers, and accumulation of an electron dense material in the cell wall. Flow ...

Source: Journal of Medical Microbiology - February 13, 2015 Category: Microbiology Authors: Borba-Santos LP, Gagini T, Ishida K, de Souza W, Rozental S Tags: J Med Microbiol Source Type: research