Time ‐based investigation of urinary metabolic markers for Type 2 diabetes: Metabolomics approach for diabetes management

The objective of this study was to monitor the time ‐course variation in the metabolic profile of diabetic rats to detect urinary metabolic biomarkers using the metabolomics approach. Type 2 diabetes was induced in male Wistar albino rats using a single intraperitoneal injection of 40 mg/kg of streptozotocin following oral administration of 10% f ructose in drinking water for 3 weeks. Then, urine was collected for 24 h from rats at three time points (0, 2, and 4 weeks after confirmation of diabetes), and were analyzed by nuclear magnetic resonance (H1‐NMR), followed by multivariate data analysis. The results from H1‐NMR pointed out thatd‐glucose, taurine,l‐carnitine,l‐fucose, 1,5‐anhydrosorbitol, andd‐galactose levels showed consistent significant variation (p<  0.05) between the positive (diabetic) and negative (normal) controls during the whole experimental period. Also, with the disease progression, myoinositol, andl‐phenylalanine levels were significantly altered (p<  0.05) after 2 weeks and this alteration was maintained till the end of the 4‐week experimental period in the positive control group. From the results of the present study, it could be concluded that we cannot depend only on glucose levels for prognostic purposes since there are other metaboli c disturbances in diabetes which need to be tracked for better disease prognosis.
Source: BioFactors - Category: Biochemistry Authors: Tags: Research Communication Source Type: research