Platelet and Neutrophil Responses to Porphyromonas gingivalis in Human Whole Blood

This study evaluates the role ofP.gingivalis on platelet expression of CD62P, platelet ‐neutrophil interactions, and labeled neutrophil associated DNA. Human whole blood was preincubated with varyingP.gingivalis concentrations with or without subsequent addition of adenosine diphosphate (ADP). Flow cytometry was employed to measure CD62P expression in platelets using PerCP ‐anti‐CD61 and PE‐anti‐CD62P, platelet‐neutrophil interactions using PerCP‐anti‐CD61 and FITC‐anti‐CD16, and the release of neutrophil DNA using FITC‐anti‐CD16 and Sytox Blue labeling. Preincubation with high (6.25 x 106 CFU/mL) level ofP.gingivalis significantly increased platelet expression of CD62P in ADP treated and untreated whole blood. In addition, after ADP stimulation, following 5 ‐ 22 min preincubation of highP.gingivalis CFU with blood, platelet ‐neutrophil interactions were significantly increased. However, in the absence of added ADP, platelet‐neutrophil interactions increased in a manner dependent on the preincubation time withP.gingivalis. Moreover, after ADP addition, 16 min preincubation of whole blood withP.gingivalis led to increased labelling of neutrophil associated DNA. Taken together, the results suggest that presence ofP.gingivalis alters platelet and neutrophil responses to increase platelet activation, platelet interactions with neutrophils, and the level of neutrophil antimicrobial NETs.
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research