Molecules, Vol. 26, Pages 1186: Constructing an Efficient Bacillus subtilis Spore Display by Using Cohesin −Dockerin Interactions

In this study, five different pairs of cohesins and dockerins, selected from four cellulolytic microbes, were examined for their capabilities in displaying a tetrameric enzyme β-galactosidase from Bacillus stearothermophilus IAM11001 on the surface of B. subtilis WB600 spores. Immunofluorescence microscopy, western blotting, dot blotting, and enzyme assay was applied to confirm its surface expression. All the resultant five Coh–Doc based spore display can hydrolyze o-nitrophenyl-β-D-galactopyranoside. Further, the optimized Coh–Doc based spore display exhibited the highest display efficiency. Overall, the results of current study may open new perspectives on the use of Coh–Doc interaction, which will find application in improving the efficiency of B. subtilis spore display.

Source: Molecules - February 23, 2021 Category: Chemistry Authors: He Wang Xiaomin Jiang Yongchang Qian Lianghong Yin Tags: Article Source Type: research