HMGA2-induced epithelial –mesenchymal transition is reversed by let-7d in intrauterine adhesions

In this study, we demonstrate that the mRNA and protein levels of high mobility group AT-hook 2 (HMGA2) were increased by nearly 3-fold (P <  0.0001) and 5-fold (P = 0.0095) in the endometrial epithelial cells (EECs) of IUA patients (n = 18) compared to controls.In vivo andin vitro models of endometrial fibrosis also confirmed the overexpression of HMGA2 in EECs.In vitro cell experiments indicated that overexpression of HMGA2 promoted the epithelial –mesenchymal transition (EMT) while knockdown of HMGA2 reversed transforming growth factor-β-induced EMT. A dual luciferase assay confirmed let-7d microRNA downregulated HMGA2 and repressed the pro-EMT effect of HMGA2in vitro andin vivo. Therefore, our data reveal that HMGA2 promotes IUA formation and suggest that let-7d can depress HMGA2 and may be a clinical targeting strategy in IUA.
Source: Molecular Human Reproduction - Category: Molecular Biology Source Type: research