Impaired decidualization of human endometrial stromal cells from women with adenomyosis.

Impaired decidualization of human endometrial stromal cells from women with adenomyosis. Biol Reprod. 2021 Feb 02;: Authors: Peng Y, Jin Z, Liu H, Xu C Abstract Differentiation of endometrial stromal cells (ESCs) into secretory decidualized cells (dESCs) is essential for embryo implantation. Adenomyosis is a common benign gynecological disease that causes infertility. However, whether adenomyosis affects decidualization of human endometrial stromal cells is elusive. Primary eutopic ESCs were obtained from patients with adenomyosis (n = 9) and women with non-endometrial diseases (n = 12). We determined the capacity of decidualization of human ESCs by qRT-PCR, Edu proliferation assay, cytokine array and ELISA assay. We found that the expression of decidualization markers (IGFBP1 and PRL) in ESCs of adenomyosis was reduced, concomitant with increased cell proliferation. Differential secretion of cytokines in dESCs, including CXCL1/2/3, IL-6, IL-8, MCP-1, VEGF-A, MIP-3α, OPN, SDF-1α, HGF and MMP-9, were observed between adenomyosis and non-adenomyosis. Moreover, the expression of decidualization regulators (HOXA10 at both mRNA and protein levels, FOXO1, KLF5, CEBPB and HAND2 at mRNA levels) in the eutopic endometrium of adenomyosis were lower than that of non-adenomyosis. We propose that ESCs from adenomyosis have defected ability to full decidualization, which may lead to a non-receptive endometrium. PMID: 33533396 [PubMed...
Source: Biology of Reproduction - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research