Comparison of oral microbiome profile of polymers modified with silver and vanadium base nanomaterial by next-generation sequencing

This study aimed to evaluate two methods of the incorporation of nanostructured silver vanadate decorated with silver nanoparticles (AgVO3) into acrylic resin and characterize the profile of early and late microbial communities in class and family taxonomic level by pyrosequencing. The specimens were made by adding different concentrations of AgVO3 (1, 2.5, and 5%) to the heat-activated acrylic resin by two methods: vacuum spatulation (VS) and polymeric film (PF). A control group (0%) without AgVO3 was also obtained for both methods. After 24  h and 7 days of incubation in human saliva, biofilm samples were collected, DNA was extracted, and 16S rRNA genes were sequenced by the 454-Roche sequencing platform. Seventeen classes and 51 families of bacteria were identified. The abundance ofBacteroidia,Bacilli,Negativicutes,Fusobacteria andBetaproteobacteria classes decreased after 7  days of incubation, andClostridia,Gammaproteobacteria, and unclassified bacteria increased. TheNegativicutes andBetaproteobacteria classes were more abundant when the PF method was used, andGammaproteobacteria was more abundant when VS was used. The incorporation of 5% AgVO3 promoted a reduction in the prevalence ofBacilli,Clostridia,Negativicutes,Betaproteobacteria, and unclassified bacteria, and increasedGammaproteobacteria. The addition of AgVO3 to acrylic resin altered the early and mature microbiome formed on the specimen surface, and the PF method presented a more favorable microbial profile ...
Source: Odontology - Category: Dentistry Source Type: research