Distinct cell death markers identified in critical care patient survivors diagnosed with sepsis.

The objective of the current study is to investigate the presence of molecular markers of relevance to apoptotic and non-apoptotic cell death in control healthy subjects and septic patient survivors. Sepsis survivors (N = 24) and healthy human volunteers (N = 16) [40 total subjects] were recruited into the study. Clinical intervention included antibiotic treatment regimen administered to patients upon clinical diagnosis of sepsis followed by blood draw 18-24 hr post-antibiotic dose. Serum samples analyzed by enzyme-linked immunosorbent assay (ELISA) and peripheral blood mononuclear cells (PBMCs) by flow cytometry analysis for identification of cell death markers. Cell death markers analyzed by ELISA and flow cytometry included caspase-1, caspase-3, MLKL, RIPK3, p62 and LC3B. Serum and peripheral blood mononuclear cells (PBMCs) of septic survivors and healthy controls analyzed for the presence of distinct cell death markers. Markers of relevance to apoptosis (caspase-3), pyroptosis (caspase-1), necroptosis (MLKL) and autophagy (p62 and LC3B) were compared between septic survivors and healthy controls. ELISA analysis suggested significant alteration in the serum levels of non-apoptotic cell death markers, Caspase-1 and p62/SQSTM1 in septic survivors compared to healthy controls (p <  0.05). There was no significant difference in the serum levels of caspase-3 and MLKL between septic survivors and healthy control subjects (p >  0.05). Intracellular levels ...

Source: Immunology Letters - January 3, 2021 Category: Allergy & Immunology Authors: Mallarpu CS, Ponnana M, Prasad S, Singarapu M, Kim J, Haririparsa N, Bratic N, Brar H, Chelluri LK, Madiraju C Tags: Immunol Lett Source Type: research