Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in < i > Mycobacterium tuberculosis < /i > –infected lungs

by Antony Fearns, Daniel J. Greenwood, Angela Rodgers, Haibo Jiang, Maximiliano G. Gutierrez Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we develope d correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type–specific accumulation of an antibiotic in lung lesions of mice infected withMycobacterium tuberculosis. Using CLEIMiT, we found that the anti-tuberculosis (TB) drug bedaquiline (BDQ) is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells.
Source: PLoS Biology: Archived Table of Contents - Category: Biology Authors: Source Type: research