Identification of novel serological autoantibodies in Takayasu arteritis patients using HuProt arrays.

Identification of novel serological autoantibodies in Takayasu arteritis patients using HuProt arrays.
Mol Cell Proteomics. 2020 Dec 17;:
Authors: Wen XT, Song G, Hu CJ, Pan J, Wu ZY, Li L, Liu C, Tian X, Zhang FC, Qian J, Zhu H, Li YZ
Abstract
To identify novel autoantibodies of Takayasu arteritis (TAK) using HuProt array-based approach. A two-phase approach was adopted. In Phase I, serum samples collected from 40 TAK patients, 15 autoimmune disease patients, and 20 healthy subjects were screened to identify TAK-specific autoantibodies using human protein (HuProt) arrays. In Phase II, the identified candidate autoantibodies were validated with TAK-focused arrays using an additional cohort comprised of 109 TAK patients, 110 autoimmune disease patients, and 96 healthy subjects. Subsequently, the TAK-specific autoantibodies validated in Phase II were further confirmed using Western blot analysis. We identified and validated eight autoantibodies as potential TAK-specific diagnostic biomarkers, including anti-SPATA7, -QDPR, -SLC25A2, -PRH2, -DIXDC1, -IL17RB, -ZFAND4, and -NOLC1 antibodies, with AUC of 0.803, 0.801, 0.780, 0.696, 0.695, 0.678, 0.635 and 0.613, respectively. SPATA7 could distinguish TAK from healthy and disease controls with 73.4% sensitivity at 85.4% specificity, while QDPR showed 71.6% sensitivity at 86.4% specificity. SLC25A22 showed the highest sensitivity of 80.7%, but at lower specificity of 67.0%. In addition, PRH2,...

Source: Molecular and Cellular Proteomics : MCP - December 17, 2020 Category: Molecular Biology Authors: Wen XT, Song G, Hu CJ, Pan J, Wu ZY, Li L, Liu C, Tian X, Zhang FC, Qian J, Zhu H, Li YZ Tags: Mol Cell Proteomics Source Type: research