Isolation and Phenotyping of Splenic Myeloid-Derived Suppressor Cells in Murine Cancer Models.

Isolation and Phenotyping of Splenic Myeloid-Derived Suppressor Cells in Murine Cancer Models. Methods Mol Biol. 2021;2236:19-28 Authors: Sanseviero E, Kim R, Gabrilovich DI Abstract Myeloid-derived suppressor cells (MDSC) are immunosuppressive myeloid cells that accumulate in tumor sites and peripheral lymphoid organs such as the spleen. In murine cancer models, the spleen is a major reservoir for MDSC, representing an easily accessible tissue from which to isolate high numbers of these cell population for downstream applications. Here we describe an efficient method to phenotype as well as to isolate and assess the functionality of murine splenic MDSC. PMID: 33237537 [PubMed - as supplied by publisher]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research