Novel orthogonal methods to uncover the complexity and diversity of nuclear architecture.

Novel orthogonal methods to uncover the complexity and diversity of nuclear architecture.
Curr Opin Genet Dev. 2020 Nov 18;67:10-17
Authors: Tjalsma SJ, de Laat W
Abstract
Recent years have seen a vast expansion of knowledge on three-dimensional (3D) genome organization. The majority of studies on chromosome topology consists of pairwise interaction data of bulk populations of cells and therefore conceals heterogenic and more complex folding patterns. Here, we discuss novel methodologies to study the variation in genome topologies between different cells and techniques that allow analysis of complex, multi-way interactions. These technologies will aid the interpretation of genome-wide chromosome conformation data and provide strategies to further dissect the interplay between genome architecture and transcription regulation.
PMID: 33220512 [PubMed - as supplied by publisher]

Source: Current Opinion in Genetics and Development - November 18, 2020 Category: Genetics & Stem Cells Authors: Tjalsma SJ, de Laat W Tags: Curr Opin Genet Dev Source Type: research