An in situ hybridization study of decorin and biglycan mRNA in mouse osteoblasts in vivo

AbstractIn situ hybridization ofdecorin andbiglycan mRNA, principal members of small leucine-rich proteoglycan, was performed using [35S]-labeled RNA probes, in the context of the hypothesis that they show different expression patterns associated with osteoblast differentiation in mice. We adopted two ossifying sites that can clearly follow the developmental process of bone formation: ossifying tympanic ring and developing bone collar of mandibular condylar cartilage.Decorin mRNA was expressed in osteoblasts of developing tympanic ring at E14.0, as well as of developing bone collar at E15.0, butbiglycan mRNA was not, indicating decorin mRNA was expressed earlier in newly differentiating osteoblasts thanbiglycan. With maturation of osteoblasts,biglycan mRNA became expressed and maintained its expression both in the outer region (periosteum) and in the interior region (endosteum) of bone. By contrast,decorin mRNA expression was maintained in the outer region but diminished in the interior region. These results indicate thatdecorin andbiglycan show differential expression patterns in differentiating osteoblasts and play specific roles in bone formation.

Source: Anatomical Science International - November 20, 2020 Category: Anatomy Source Type: research