The Saccharomyces  cerevisiae ABC subfamily D transporter Pxa1/Pxa2p co‐imports CoASH into the peroxisome

ATP ‐binding cassette (ABC) subfamily D transporters are important for the uptake of fatty acids and other beta‐oxidation substrates into peroxisomes. Genetic and biochemical evidence indicates that the transporters accept fatty acyl‐coenzyme A that is cleaved during the transport cycle and then r e‐esterified in the peroxisomal lumen. However, it is not known whether free coenzyme A (CoA) is released inside or outside the peroxisome. Here we have usedSaccharomyces  cerevisiae and isolated peroxisomes to demonstrate that free CoA is released in the peroxisomal lumen. Thus, ABC subfamily D transporter provide an import pathway for free CoA that controls peroxisomal CoA homeostasis and tunes metabolism according to the cell ’s demands.

Source: FEBS Letters - November 11, 2020 Category: Biochemistry Authors: Carlo W. T. Roermund, Lodewijk IJlst, Alison Baker, Ronald J. A. Wanders, Freddie L. Theodoulou, Hans R. Waterham Tags: Research Article Source Type: research