Validation of the International Space Station Smart Sample Concentrator for Microbial Monitoring of Low Biomass Water Samples.

This report presents the data from a validation study of a NASA-sponsored small business research initiative to develop a microgravity-compatible, automated water concentrator to be used on the ISS, which has been named the ISS Smart Sample Concentrator (iSSC). Efficiency and reproducibility of the iSSC were compared with a ground-based automated water concentrator and the standard Millipore manual filtration. Using 104 CFU/L of Sphingomonas paucimobilis, Ralstonia pickettii, and Cupriavidus basilensis and a mixed microbial community, we have shown, through culture and qPCR, that the iSSC is comparable, if not better, at recovering and concentrating bacteria from large volumes of water, with good reproducibility. PMID: 33100920 [PubMed - as supplied by publisher]
Source: Journal of Biomolecular Techniques : JBT - Category: Molecular Biology Authors: Tags: J Biomol Tech Source Type: research