Emerging mass spectrometry-based proteomics methodologies for novel biomedical applications.

Emerging mass spectrometry-based proteomics methodologies for novel biomedical applications.
Biochem Soc Trans. 2020 Oct 20;:
Authors: Pino LK, Rose J, O'Broin A, Shah S, Schilling B
Abstract
Research into the basic biology of human health and disease, as well as translational human research and clinical applications, all benefit from the growing accessibility and versatility of mass spectrometry (MS)-based proteomics. Although once limited in throughput and sensitivity, proteomic studies have quickly grown in scope and scale over the last decade due to significant advances in instrumentation, computational approaches, and bio-sample preparation. Here, we review these latest developments in MS and highlight how these techniques are used to study the mechanisms, diagnosis, and treatment of human diseases. We first describe recent groundbreaking technological advancements for MS-based proteomics, including novel data acquisition techniques and protein quantification approaches. Next, we describe innovations that enable the unprecedented depth of coverage in protein signaling and spatiotemporal protein distributions, including studies of post-translational modifications, protein turnover, and single-cell proteomics. Finally, we explore new workflows to investigate protein complexes and structures, and we present new approaches for protein-protein interaction studies and intact protein or top-down MS. While these approaches are only rece...

Source: Biochemical Society Transactions - October 20, 2020 Category: Biochemistry Authors: Pino LK, Rose J, O'Broin A, Shah S, Schilling B Tags: Biochem Soc Trans Source Type: research