Tylophora hirsuta (Wall.) extracts ameliorates diabetes associated with inflammation in alloxan induced diabetic rats.

Tylophora hirsuta (Wall.) extracts ameliorates diabetes associated with inflammation in alloxan induced diabetic rats. Endocr Metab Immune Disord Drug Targets. 2020 Aug 21;: Authors: Razzaque F, Sharif A, Akhtar B, Khan HM, Akhtar MF, Zaib M, Muhammad A, Sohail K, Hamid I, Qaisar N Abstract BACKGROUND: Tylophora hirsuta Wall. has long been used as traditional medicine for the treatment of diabetes. Current study is designed to evaluate the antidiabetic and anti-inflammatory activity of different extracts of aerial parts of Tylophora hirsuta. METHODS: Sequential maceration was conducted to obtain extracts. Total phenolic contents were determined by FolinCiocalteau method. The antioxidant activity was assessed by DPPH free radical scavenging assay. The extracts were tested for its inhibitory activity against α-amylase in-vitro. In-vivo anti diabetic assay was conducted using alloxan induced diabetic model and OGTT was conducted on normal rats. ELISA was used to determine the proinflammatory cytokines (TNF-α and IL-6). Polyphenolic composition of the extract was analyzed using a HPLC system. RESULTS: Aqueous extract exhibited highest total phenolic contents (985.24± 3.82 mg GAE/100 g DW), antioxidant activity (IC50 = 786.70 ± 5.23. PMID: 32955006 [PubMed - as supplied by publisher]
Source: Endocrine, Metabolic and Immune Disorders Drug Targets - Category: Drugs & Pharmacology Tags: Endocr Metab Immune Disord Drug Targets Source Type: research