Methylococcus geothermalis sp. nov., a methanotroph isolated from a geothermal field in the Republic of Korea.

Methylococcus geothermalis sp. nov., a methanotroph isolated from a geothermal field in the Republic of Korea.
Int J Syst Evol Microbiol. 2020 Sep 10;:
Authors: Awala SI, Bellosillo LA, Gwak JH, Nguyen NL, Kim SJ, Lee BH, Rhee SK
Abstract
A Gram-stain-negative, aerobic, non-motile and coccoid methanotroph, strain IM1T, was isolated from hot spring soil. Cells of strain IM1T were catalase-negative, oxidase-positive and displayed a characteristic intracytoplasmic membrane arrangement of type I methanotrophs. The strain possessed genes encoding both membrane-bound and soluble methane monooxygenases and grew only on methane or methanol. The strain was capable of growth at temperatures between 15 and 48 °C (optimum, 30-45 °C) and pH values between pH 4.8 and 8.2 (optimum, pH 6.2-7.0). Based on phylogenetic analysis of 16S rRNA gene and PmoA sequences, strain IM1T was demonstrated to be affiliated to the genus Methylococcus. The 16S rRNA gene sequence of this strain was most closely related to the sequences of an uncultured bacterium clone FD09 (100 %) and a partially described cultured Methylococcus sp. GDS2.4 (99.78 %). The most closely related taxonomically described strains were Methylococcus capsulatus TexasT (97.92 %), Methylococcus capsulatus Bath (97.86 %) and Methyloterricola oryzae 73aT (94.21 %). Strain IM1T shared average nucleotide identity values of 85.93 and 85.62 % with Methylococcus capsulatus strains Tex...

Source: International Journal of Systematic and Evolutionary Microbiology - September 9, 2020 Category: Microbiology Authors: Awala SI, Bellosillo LA, Gwak JH, Nguyen NL, Kim SJ, Lee BH, Rhee SK Tags: Int J Syst Evol Microbiol Source Type: research