Heat-Shock Protein 90 (HSP90) in Age-Dependent Changes in the Fibroblast Number in Human Skin

AbstractThe goal of this work was to study the content of heat-shock protein 90 (HSP90) in fibroblasts of the human dermis, from embryonic development to extreme age (from 20 weeks of gestation to 85 years old), and to determine the role of HSP90 in age-dependent changes in fibroblast number in the human dermis. HSP90 and proliferating cell nuclear antigen (PCNA) were detected in skin sections with the indirect immunohistochemical method. The results showed that the proportion of dermal fibroblasts with the positive staining for HSP90 remains constant in human skin from 20 weeks of gestation to 20 years old. From the age of 21 to 60 years, a systematic decrease in the proportion of HSP90 positive fibroblasts in the dermis is observed. In people 61 –85 years old, there is a sharp increase in the proportion of dermal fibroblasts with positive staining for HSP90. Age-dependent changes in the content of HSP90 positive fibroblasts in the dermis are not statistically associated with an age-related decrease in the total number or proportion of PCN A positive fibroblasts in the dermis.
Source: Advances in Gerontology - Category: Geriatrics Source Type: research