MicroRNA-7 Negatively Regulates Toll-Like Receptor 4 Signaling Pathway through FAM177A.

MicroRNA-7 Negatively Regulates Toll-Like Receptor 4 Signaling Pathway through FAM177A. Immunology. 2020 Aug 27;: Authors: Chen H, Guo M, Yue D, Zhao J, Zhou Y, Chen C, Liang G, Xu L Abstract Toll-like receptor (TLR) 4 signaling is critical for innate immunoinflammatory response and widely triggers the development of various types of clinical diseases. MicroRNA-7 (miR-7) is well documented plays an important regulator role in various biological events. However, the exact role of miR-7 in TLR4 signaling pathway remains to be fully elucidated. In present study, we found that miR-7 expression in TLR4 signaling-activated bone marrow-derived macrophages (BMDMs) stimulated by LPS dramatically increased. Importantly, miR-7 deficiency significantly enhanced the production of related inflammatory cytokines including IL-1β, IL-6 and IL-12, as well as TNF-α, on LPS-activated BMDMs, accompanied by elevated transduction of TLR4 signaling including Myd88 dependent and independent pathway. Whereas, miR-7 overexpression significantly decreased the transduction of TLR4 signaling and the production of related inflammatory cytokines. Mechanistically, we identified family with sequence similarity 177, member A (FAM177A) as a novel target molecule of miR-7. Furthermore, down-regulation of FAM177A using RNAi could impair the transduction of TLR4 signaling. Finally, down-regulation of FAM177A also reversed the effect of miR-7 deficiency on TLR4 signaling...
Source: Immunology - Category: Allergy & Immunology Authors: Tags: Immunology Source Type: research