Enhancing native chemical ligation for challenging chemical protein syntheses.

Enhancing native chemical ligation for challenging chemical protein syntheses.
Curr Opin Chem Biol. 2020 Jul 31;58:37-44
Authors: Giesler RJ, Erickson PW, Kay MS
Abstract
Native chemical ligation has enabled the chemical synthesis of proteins for a wide variety of applications (e.g., mirror-image proteins). However, inefficiencies of this chemoselective ligation in the context of large or otherwise challenging protein targets can limit the practical scope of chemical protein synthesis. In this review, we focus on recent developments aimed at enhancing and expanding native chemical ligation for challenging protein syntheses. Chemical auxiliaries, use of selenium chemistry, and templating all enable ligations at otherwise suboptimal junctions. The continuing development of these tools is making the chemical synthesis of large proteins increasingly accessible.
PMID: 32745915 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - July 30, 2020 Category: Biochemistry Authors: Giesler RJ, Erickson PW, Kay MS Tags: Curr Opin Chem Biol Source Type: research