Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach.

Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach. Biofouling. 2020 Jul 26;:1-15 Authors: Romeu MJL, Domínguez-Pérez D, Almeida D, Morais J, Campos A, Vasconcelos V, Mergulhão FJM Abstract Cyanobacteria promote marine biofouling with significant impacts. A qualitative proteomic analysis, by LC-MS/MS, of planktonic and biofilm cells from two cyanobacteria was performed. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s-1 and 40 s-1). For both strains and surfaces, biofilm development was higher at 4 s-1. Biofilm development of Nodosilinea sp. LEGE 06145 was substantially higher than Nodosilinea sp. LEGE 06119, but no significant differences were found between surfaces. Overall, 377 and 301 different proteins were identified for Nodosilinea sp. LEGE 06145 and Nodosilinea sp. LEGE 06119. Differences in protein composition were more noticeable in biofilms formed under different hydrodynamic conditions than in those formed on different surfaces. Ribosomal and photosynthetic proteins were identified in most conditions. The characterization performed gives new insights into how shear rate and surface affect the planktonic to biofilm transition, from a structural and proteomics perspective. PMID: 32715767 [PubMed - as supplied by publisher]
Source: Biofouling - Category: Molecular Biology Authors: Tags: Biofouling Source Type: research